Detailed information of TA system TA168889

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 5317873..5318530
• Replicon: chromosome
• Accession: NZ_CP059468
• Organism: Klebsiella pneumoniae strain BA25665

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: HBJ46_RS25755
• Protein ID: WP_004181233.1
• Coordinates: 5317873..5318283 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: HBJ46_RS25760
• Protein ID: WP_002916312.1
• Coordinates: 5318264..5318530 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBJ46_RS25735 (5313873)	5313873..5315606	-	1734	WP_004149758.1	single-stranded-DNA-specific exonuclease RecJ	-
HBJ46_RS25740 (5315612)	5315612..5316325	-	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HBJ46_RS25745 (5316348)	5316348..5317244	-	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
HBJ46_RS25750 (5317345)	5317345..5317866	+	522	WP_004144730.1	flavodoxin FldB	-
HBJ46_RS25755 (5317873)	5317873..5318283	-	411	WP_004181233.1	protein YgfX	Toxin
HBJ46_RS25760 (5318264)	5318264..5318530	-	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
HBJ46_RS25765 (5318776)	5318776..5319759	+	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
HBJ46_RS25770 (5319910)	5319910..5320569	-	660	WP_002916317.1	hemolysin III family protein	-
HBJ46_RS25775 (5320733)	5320733..5321044	-	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
HBJ46_RS25780 (5321094)	5321094..5321822	+	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
HBJ46_RS25785 (5321941)	5321941..5323374	+	1434	WP_004181234.1	6-phospho-beta-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16121.92 Da
• Isoelectric Point: 11.1565

>T168889 WP_004181233.1 NZ_CP059468:c5318283-5317873 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAEEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T168889 NZ_CP076253:c1378496-1378203 [Xanthomonas translucens pv. graminis]
ATGACCGAAAACCTGACCACCTACGATCCTGCCGAGGATCTGAACTCCGACCAGGCGATTGCCGATTTCATGGCGGGCGC
CTTTGCGACCAACGACACCGGCTTCATCGCCCATGCCCTCGGCATCGTCGCCCGTGCCAAGGGCATGACCCAGATCGCAC
GACAAACCGGCCTGTCCCGGGAGCAGCTCTACCGCTCCTTCAGCGAGGACGGAAATCCGACGCTGCGCACCACCCTGGCC
GTCATGAAGGCGCTCGGGATCGAACTGACGGCCAAAGCCGCCGTCACGCATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT168889 WP_002916312.1 NZ_CP059468:c5318530-5318264 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT168889 NZ_CP076253:c1378795-1378499 [Xanthomonas translucens pv. graminis]
GTGATCGAACTCAAGCAGACCGAGACGTTCCGCAAGTGGCGCACGAAGCTGAAGGACGAGCGCGCTCGCACTGCGATTGC
CTTGCGCTTGACCCGACTTGCCTATGGCCATGCCGGCGATACGGAAGCTGTCGGGCAAGGCATCAGCGAGCTGCGTATCC
ACCATGGCCCCGGATATCGGGTGTATTTCCAGCGGCGCGGCGACACGATCATCGTCTTGCTCTGCGGTGGCGACAAAAGC
TCGCAGGCAAAGGACATCAAGACAGCCAAACGGCTTGCCGACGAATGGAGCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GY41