Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1351660..1352470 Replicon chromosome
Accession NZ_CP059468
Organism Klebsiella pneumoniae strain BA25665

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag HBJ46_RS06435 Protein ID WP_004178461.1
Coordinates 1351937..1352470 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HBJ46_RS06430 Protein ID WP_002887278.1
Coordinates 1351660..1351926 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HBJ46_RS06410 (1347532) 1347532..1347876 - 345 WP_004197273.1 cation efflux system protein CusF -
HBJ46_RS06415 (1347894) 1347894..1349279 - 1386 WP_004178457.1 efflux transporter outer membrane subunit -
HBJ46_RS06420 (1349451) 1349451..1350134 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
HBJ46_RS06425 (1350124) 1350124..1351557 + 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
HBJ46_RS06430 (1351660) 1351660..1351926 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HBJ46_RS06435 (1351937) 1351937..1352470 + 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
HBJ46_RS06440 (1352518) 1352518..1353639 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T168877 WP_004178461.1 NZ_CP059468:1351937-1352470 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T168877 NZ_CP076251:685443-685919 [Xanthomonas translucens pv. phlei]
TTGTCGCTCACTGTTCCCGGCCCGCTCAACGACGTGCATCGGCTGGACGACTTCCAGTGCAGTGCACCGGAACTGACAAG
ATGGCTGCTGGAACGAGCGCACCAGAATCAGCTATCCGGCGCATCTAGGTGCTTCGTCGTCTGCGACGGACAGCAGCGCG
TTGTGGGCTACTACGCACTGGCGGCCGGTGTGGTGGCGCATGAAGACGCGCCTGGCCGCATCCATCGCAACATGCCCAAT
CCCGTTCCGGTGATCGTCCTGGGGCGTCTGGCGGTGCACGTCGACTGGGCCGGCCAGGGCGTCGGCAGGGGATTGCTCAA
GGACGCCGTACAACGAACGTTGCAGGTCTGCGAGCAGGTGGGCGCCCGTGCGCTGCTGTGTCACGCCATCGATGAAGCAG
CGAAGGCGTTCTATCTCAAACACGGCTTCATCGCCTCGCCCATCCACGATAGGACGGTGATGTTGCCGCTTAGGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT168877 WP_002887278.1 NZ_CP059468:1351660-1351926 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT168877 NZ_CP076251:685168-685440 [Xanthomonas translucens pv. phlei]
ATGAGCGCCGTCACCAGCAAAGTCATCAATTTTCGGGCGCCTGCTGCCAAACAGGCGCTGATCGACCACGCCGTGGAAGT
CAGCGGCATGAACCGCACAGAGTTCATCCTGGACGCCGTGTGCGAGAAGGCGCGCGAGGTGCTGGCCGACCAGACCCGAT
TCGCCCTGAGCCCGCAGCAGCTGCAGCGCTTCAATGCGCTACTCGATGCGCCTCTGGAGAGTAATGCAGCGATCCGTCGC
CTGTTGAGCACGCCGGCGCCTTGGGAACACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References