Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 772835..773460 Replicon chromosome
Accession NZ_CP059468
Organism Klebsiella pneumoniae strain BA25665

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HBJ46_RS03765 Protein ID WP_002882817.1
Coordinates 773077..773460 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HBJ46_RS03760 Protein ID WP_004150355.1
Coordinates 772835..773077 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HBJ46_RS03735 (768826) 768826..769425 + 600 WP_004151865.1 glucose-1-phosphatase -
HBJ46_RS03740 (769419) 769419..770279 + 861 WP_004146232.1 virulence factor BrkB family protein -
HBJ46_RS03745 (770276) 770276..770713 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HBJ46_RS03750 (770758) 770758..771699 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HBJ46_RS03755 (771713) 771713..772630 - 918 WP_004181612.1 alpha/beta hydrolase -
HBJ46_RS03760 (772835) 772835..773077 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HBJ46_RS03765 (773077) 773077..773460 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HBJ46_RS03770 (773634) 773634..774563 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HBJ46_RS03775 (774560) 774560..775195 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HBJ46_RS03780 (775192) 775192..776094 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T168874 WP_002882817.1 NZ_CP059468:773077-773460 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T168874 NZ_CP076250:629503-629697 [Xanthomonas translucens pv. poae]
ATGCCCCCGAAAATCCGCGACGTGCTGCAACAACTCAGGGACGCAGGCTGGCAGCAAGTCGGCCAGACCGGCAGCCACCG
CCAGTTCAAGCATCCTGTCTACCCAGGCCGCGTGACAGTAGCCGGCAAGCCGAGCGATGATGTGGCTCCCGGCACCCTGA
ACAGCATCCTGAAGCAGGCCGGCCTGAAGAGGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT168874 WP_004150355.1 NZ_CP059468:772835-773077 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT168874 NZ_CP076250:629703-629915 [Xanthomonas translucens pv. poae]
ATGCGCTACGCCGTCGTTATTGAGCAAGCGGGCAACAACTACTCCGCGTACGTGCCGGATCTGCCGGGGTGTGTGGCGAC
GGGCGCGACGACTGCGGAGGTGGAAGGTTTGATCCGCGAAGCGATCGAGCTGCATCTTGCTGGGATGCGTGAGGACGGGG
AAGTGATTCCGCTGCCGTCGAGTCAGGTGGAGTATGTGGATGTGGCAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References