168858

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA(toxin)
Location 3685047..3685660 Replicon chromosome
Accession NZ_CP059448
Organism Pseudomonas fluorescens strain 2020WEIHUA_J

Toxin (Protein)


Gene name hicA Uniprot ID A0A172Z267
Locus tag H1Q68_RS16530 Protein ID WP_053256594.1
Coordinates 3685047..3685298 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1E4XBZ6
Locus tag H1Q68_RS16535 Protein ID WP_053256595.1
Coordinates 3685295..3685660 (+) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1Q68_RS16530 (H1Q68_16495) 3685047..3685298 + 252 WP_053256594.1 type II toxin-antitoxin system HicA family toxin Toxin
H1Q68_RS16535 (H1Q68_16500) 3685295..3685660 + 366 WP_053256595.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H1Q68_RS16540 (H1Q68_16505) 3685643..3686833 - 1191 WP_053256596.1 MFS transporter -
H1Q68_RS16545 (H1Q68_16510) 3686976..3687569 + 594 WP_053256597.1 TetR/AcrR family transcriptional regulator -
H1Q68_RS16550 (H1Q68_16515) 3687553..3688908 - 1356 WP_053256598.1 MATE family efflux transporter -
H1Q68_RS16555 (H1Q68_16520) 3688934..3689506 - 573 WP_053256599.1 DUF2239 family protein -
H1Q68_RS16560 (H1Q68_16525) 3689547..3690530 - 984 WP_053256600.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-76)

Antitoxin

Uncharacterised protein family HicB

(55-105)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9485.04 Da        Isoelectric Point: 9.6594

>T168858 WP_053256594.1 NZ_CP059448:3685047-3685298 [Pseudomonas fluorescens]
VSKNEKLLAKLLNEHMAFTWPELVTLLHRLGYTQIEGAGSRVKFDIGDPSAMISLHKPHPGNELKHYIRRQIIEQLKSGE
LIQ

Download         Length: 252 bp

>T168858 NZ_CP076248:c1068952-1068695 [Xanthomonas translucens pv. hordei]
GTGACGCTTCAGTTCTCGGACAATGCCTGGGAGGACGACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAGCGCAT
CAACGACCTGATCAAAGCCATTCAACGCGATCCCTTCCAGGGCGTCGGCAAGCCAGAACGGCTACGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACACCGCATCGTCTACAAGGTCGAGGCCGGCATGCTGCTGATCGCGCAGGTG
CGCTACCACTACGTGTAG

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13215.09 Da        Isoelectric Point: 6.6270

>AT168858 WP_053256595.1 NZ_CP059448:3685295-3685660 [Pseudomonas fluorescens]
VNNQLKYNGYIGSIEASLEDNCLFGKILFIKALVSYEGKTVAELDAAFREAVDDYLATCHALGQTPEKPCKGSFNVRVGH
DLHLAAALAATRKKVTLNDLTRQALNEFLQHHCAEPLSVLG

Download         Length: 366 bp

>AT168858 NZ_CP076248:c1069200-1068949 [Xanthomonas translucens pv. hordei]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTTGCGGACACCATGGAGCGTGTCGTCAACAATCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTGGAGGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTGCGCAGCCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTAGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A172Z267

Antitoxin

Source ID Structure
AlphaFold DB A0A1E4XBZ6

References