Detailed information of TA system TA168854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 29408..30279
• Replicon: plasmid pCFF_09A980_P1
• Accession: NZ_CP059446
• Organism: Campylobacter fetus strain CFF09A980

Toxin (Protein)

• Gene name: Fic
• Uniprot ID: -
• Locus tag: GZ984_RS09355
• Protein ID: WP_181913241.1
• Coordinates: 29638..30279 (+)
• Length: 214 a.a.

Antitoxin (Protein)

• Gene name: Fti
• Uniprot ID: -
• Locus tag: GZ984_RS09350
• Protein ID: WP_181913240.1
• Coordinates: 29408..29641 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZ984_RS09310	24570..24941	+	372	WP_002850989.1	hypothetical protein	-
GZ984_RS09315	25052..25738	+	687	WP_181913252.1	AAA family ATPase	-
GZ984_RS09320	25741..25950	+	210	WP_181913253.1	hypothetical protein	-
GZ984_RS09325	26030..27592	-	1563	WP_181913254.1	relaxase/mobilization nuclease domain-containing protein	-
GZ984_RS09330	27567..27935	-	369	WP_181913238.1	DNA transfer protein	-
GZ984_RS09335	28150..28365	+	216	WP_046037533.1	hypothetical protein	-
GZ984_RS09340	28358..28633	+	276	WP_046037534.1	type II toxin-antitoxin system YafQ family toxin	-
GZ984_RS09345	28630..28980	+	351	WP_181913239.1	hypothetical protein	-
GZ984_RS09350	29408..29641	+	234	WP_181913240.1	hypothetical protein	Antitoxin
GZ984_RS09355	29638..30279	+	642	WP_181913241.1	Fic family protein	Toxin
GZ984_RS09360	30279..30764	+	486	WP_181913242.1	signal peptidase I	-
GZ984_RS09365	30757..31152	+	396	Protein_41	conjugal transfer protein TraM	-
GZ984_RS09370	31832..32662	+	831	WP_181913243.1	hypothetical protein	-
GZ984_RS09375	32659..33069	+	411	WP_181913244.1	PIN domain-containing protein	-
GZ984_RS09380	33413..34354	+	942	WP_035171013.1	P-type conjugative transfer ATPase TrbB	-
GZ984_RS09385	34368..34661	+	294	WP_065844047.1	TrbC/VirB2 family protein	-
GZ984_RS09390	34658..34948	+	291	WP_181912975.1	VirB3 family type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..52345	52345

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (46-157)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 214 a.a.
• Molecular weight: 24793.49 Da
• Isoelectric Point: 9.4419

>T168854 WP_181913241.1 NZ_CP059446:29638-30279 [Campylobacter fetus]
VNDIFEELITNNKLGIKNYDEFEKISRKYTANRIFEIQKNPIKGNFDYQHLKDTHKFVFQDVFEWAGKDRAELGLLDTFA
KRSPFNPNHIQNFVPGKELEKYSKIIFDELKEQNYLKNSKDLNTFTKGMANFLMELNALHPFREGNGRTGRIFCNELAKN
AGYKMDLNLIPKEKLITASIEAMECKNAKLEALIKANLKIFKQNLTKEQGISF

Nucleotide

• Length: 642 bp

>T168854 NZ_CP076245:c4957749-4957576 [Escherichia coli O157:H7]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 9021.18 Da
• Isoelectric Point: 4.6535

>AT168854 WP_181913240.1 NZ_CP059446:29408-29641 [Campylobacter fetus]
MKNYPEVYSLEESLEILKKYRNEISKDDYDNIKSVIGSHAIESQYLNEIDIEMLVDKAINNRTTEESINRLKNKGLL

Nucleotide

• Length: 234 bp

>AT168854 NZ_CP076245:c4958073-4957777 [Escherichia coli O157:H7]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG