Detailed information of TA system TA168853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 23166..24054
• Replicon: plasmid pCFF_09A980_P1
• Accession: NZ_CP059446
• Organism: Campylobacter fetus strain CFF09A980

Toxin (Protein)

• Gene name: Fic
• Uniprot ID: -
• Locus tag: GZ984_RS09300
• Protein ID: WP_181913251.1
• Coordinates: 23398..24054 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: Fti
• Uniprot ID: -
• Locus tag: GZ984_RS09295
• Protein ID: WP_181912979.1
• Coordinates: 23166..23414 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZ984_RS09280	18305..20494	+	2190	WP_181912981.1	DUF1738 domain-containing protein	-
GZ984_RS09285	20504..22330	+	1827	WP_065839001.1	type IV secretory system conjugative DNA transfer family protein	-
GZ984_RS09290	22342..23049	+	708	WP_181913250.1	conjugal transfer protein TraL	-
GZ984_RS09295	23166..23414	+	249	WP_181912979.1	hypothetical protein	Antitoxin
GZ984_RS09300	23398..24054	+	657	WP_181913251.1	Fic family protein	Toxin
GZ984_RS09305	24115..24573	+	459	WP_059435589.1	S26 family signal peptidase	-
GZ984_RS09310	24570..24941	+	372	WP_002850989.1	hypothetical protein	-
GZ984_RS09315	25052..25738	+	687	WP_181913252.1	AAA family ATPase	-
GZ984_RS09320	25741..25950	+	210	WP_181913253.1	hypothetical protein	-
GZ984_RS09325	26030..27592	-	1563	WP_181913254.1	relaxase/mobilization nuclease domain-containing protein	-
GZ984_RS09330	27567..27935	-	369	WP_181913238.1	DNA transfer protein	-
GZ984_RS09335	28150..28365	+	216	WP_046037533.1	hypothetical protein	-
GZ984_RS09340	28358..28633	+	276	WP_046037534.1	type II toxin-antitoxin system YafQ family toxin	-
GZ984_RS09345	28630..28980	+	351	WP_181913239.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..52345	52345

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (50-158)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25181.09 Da
• Isoelectric Point: 10.1737

>T168853 WP_181913251.1 NZ_CP059446:23398-24054 [Campylobacter fetus]
MKESNEELFKKLIADNKLGIKDEKLFDKESRKYTLVRTKEIIKTPIKGNFDYQHLKNIHKYIFQDVFHWAGKDRMEVGLH
GNFGKYAPNGTITNFVPGKDLNATAQQIFTWLKEDNYLKNSKDLNDFAKNLAEFARNLNALHPFREGNGRTQRIFLNELT
KNVGYKLDLNLIPKNKMIMASVEASQLKLGKLEALIKTNLKSFKQNLEKENTLGLSLC

Nucleotide

• Length: 657 bp

>T168853 NZ_CP076245:c4860935-4860585 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9614.88 Da
• Isoelectric Point: 4.3896

>AT168853 WP_181912979.1 NZ_CP059446:23166-23414 [Campylobacter fetus]
MTYPEVHSLEESLAILKKYKDDVSKKDYEEIKSTICGHAIEDIFANEEDIIMLVKMSTYNLSSDEILAEYKEKGFVEYER
KQ

Nucleotide

• Length: 249 bp

>AT168853 NZ_CP076245:c4861180-4860929 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA