Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) fic-relB/Fic-Fic
Location 20433..22189 Replicon plasmid pCFViADRI1362_P1
Accession NZ_CP059433
Organism Campylobacter fetus strain CFViADRI1362

Toxin (Protein)


Gene name Fic Uniprot ID C5H2Y4
Locus tag GZ989_RS11145 Protein ID WP_002850956.1
Coordinates 20433..21353 (-) Length 307 a.a.

Antitoxin (Protein)


Gene name Fic Uniprot ID B2CG46
Locus tag GZ989_RS11150 Protein ID WP_002850958.1
Coordinates 21353..22189 (-) Length 279 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZ989_RS11115 16530..17072 - 543 WP_024305482.1 hypothetical protein -
GZ989_RS11120 17289..17525 + 237 WP_024305481.1 hypothetical protein -
GZ989_RS11125 17533..17913 + 381 WP_024305480.1 hypothetical protein -
GZ989_RS11130 18165..19265 - 1101 WP_010404252.1 transposase -
GZ989_RS11135 19243..19698 - 456 WP_010404250.1 IS200/IS605 family transposase -
GZ989_RS11140 19911..20219 - 309 WP_024305489.1 hypothetical protein -
GZ989_RS11145 20433..21353 - 921 WP_002850956.1 Fic family protein Toxin
GZ989_RS11150 21353..22189 - 837 WP_002850958.1 Fic family protein Antitoxin
GZ989_RS11155 22193..22495 - 303 WP_080582791.1 DUF86 domain-containing protein -
GZ989_RS11160 22617..22928 - 312 WP_002850963.1 nucleotidyltransferase domain-containing protein -
GZ989_RS11165 23114..23569 + 456 WP_010404250.1 IS200/IS605 family transposase -
GZ989_RS11170 23547..24647 + 1101 WP_010404252.1 transposase -
GZ989_RS11175 24631..26157 - 1527 WP_191002478.1 relaxase/mobilization nuclease domain-containing protein -
GZ989_RS11180 26132..26500 - 369 WP_024305349.1 DNA transfer protein -
GZ989_RS11185 26723..26938 + 216 WP_024305348.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..40588 40588
- inside IScluster/Tn - - 19243..23569 4326


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(91-202)

Antitoxin

Fido domain

(85-182)


Sequences

Toxin        


Download         Length: 307 a.a.        Molecular weight: 35445.26 Da        Isoelectric Point: 9.2339

>T168844 WP_002850956.1 NZ_CP059433:c21353-20433 [Campylobacter fetus]
MQEQYTEIKDSNIINKQNIDIAVGLGLVDDLKPSEYFYKAVEKSRTYNELEDNVKKYYDGKKLDKKEVGEKECDIVSVNI
AKYLEKSGFTLSPVTLKTIHKNLFWDAFPQGLEKYVGVFRDVNISKKEEVLGGEKSVEYGNYDELGDMLDYDFDREKRKD
YAKMTRQEQALNVGKFVSGVWQIHPFREGNTRTIAVFTIKYLQSKGFEANNDIFKENSKYFRDALVLANYDNMKENIKSD
FSYLESFFNKFILNKNIELKLLPNSPKGHKSEKSVLSRLGAAYDKKVQSGAIKQPQISKKSKEKER

Download         Length: 921 bp

>T168844 NZ_CP076245:2950775-2950882 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 279 a.a.        Molecular weight: 31851.33 Da        Isoelectric Point: 8.5962

>AT168844 WP_002850958.1 NZ_CP059433:c22189-21353 [Campylobacter fetus]
MDGGVNLGNFLKNNNERDYWLDVCVRMAHHSTAIEGNTLSQDETASILLDGYIAKATSEREFYEVKNYRQILPKMFSSLQ
EKAKIDEKLIKDFHRLIMNNLIDNNGKFKTIENLVVGANFEPTKPYLVPVAIKDMCDNLYFRLDNAKNDDDKLKAILQSH
IKFEKIHPFSDGNGRTGRIIMIYGCLENNLAPIIIPKEQKNRYIAILRNNDIDGFMAFAKEIEKDEISRRNKFLSQRPNE
QDNSKSVLSRLGNAYAKKLESGAIKALNDTANSKEKER

Download         Length: 837 bp

>AT168844 NZ_CP076245:c2950728-2950668 [Escherichia coli O157:H7]
TGTCTGGTTTCAAGATTAGCCCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D7L3Y5

Antitoxin

Source ID Structure
AlphaFold DB A0A7D7Q668

References