Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 217213..217739 Replicon plasmid p49790_MCR
Accession NZ_CP059425
Organism Enterobacter hormaechei strain ENCL49790

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag H1Z82_RS25455 Protein ID WP_000323025.1
Coordinates 217213..217500 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag H1Z82_RS25460 Protein ID WP_000534858.1
Coordinates 217500..217739 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1Z82_RS25425 212783..212959 - 177 WP_001371930.1 hypothetical protein -
H1Z82_RS25430 213470..214414 + 945 WP_000778029.1 DUF5417 domain-containing protein -
H1Z82_RS25435 214510..215112 + 603 WP_012695474.1 hypothetical protein -
H1Z82_RS25440 215172..215522 + 351 WP_000743059.1 hypothetical protein -
H1Z82_RS25445 216054..216374 + 321 WP_000332796.1 hypothetical protein -
H1Z82_RS25450 216983..217141 - 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
H1Z82_RS25455 217213..217500 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
H1Z82_RS25460 217500..217739 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
H1Z82_RS25465 218002..218925 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
H1Z82_RS25470 219125..219697 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
H1Z82_RS25475 220173..221411 - 1239 WP_000219087.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / catA2 / aadA2 / qacE / sul1 / qnrA1 / dfrA19 / aph(3'')-Ib / aph(6)-Id / mcr-9 / tet(D) / aac(6')-IIc / ere(A) / sul2 / blaSHV-12 - 1..302836 302836


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T168842 WP_000323025.1 NZ_CP059425:c217500-217213 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T168842 NZ_CP076245:c2507479-2507201 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT168842 WP_000534858.1 NZ_CP059425:c217739-217500 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT168842 NZ_CP076245:c2507672-2507481 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References