Detailed information of TA system TA168841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 93742..95365
• Replicon: plasmid p49790_MCR
• Accession: NZ_CP059425
• Organism: Enterobacter hormaechei strain ENCL49790

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: C1IUI3
• Locus tag: H1Z82_RS24775
• Protein ID: WP_000088044.1
• Coordinates: 94040..95365 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6M3PBR7
• Locus tag: H1Z82_RS24770
• Protein ID: WP_012477377.1
• Coordinates: 93742..94035 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1Z82_RS24750	89541..89933	+	393	WP_000341066.1	hypothetical protein	-
H1Z82_RS24755	90592..91248	+	657	WP_000462754.1	trypsin-like serine protease	-
H1Z82_RS24760	91451..91948	+	498	WP_000340139.1	membrane protein	-
H1Z82_RS24765	91953..93341	+	1389	WP_012695445.1	hypothetical protein	-
H1Z82_RS24770	93742..94035	+	294	WP_012477377.1	helix-turn-helix transcriptional regulator	Antitoxin
H1Z82_RS24775	94040..95365	+	1326	WP_000088044.1	type II toxin-antitoxin system HipA family toxin	Toxin
H1Z82_RS24780	95426..95632	+	207	WP_000134171.1	hypothetical protein	-
H1Z82_RS24785	95733..96143	+	411	WP_000985909.1	hypothetical protein	-
H1Z82_RS24790	96156..96584	+	429	Protein_92	hypothetical protein	-
H1Z82_RS24795	96862..97866	-	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-
H1Z82_RS24800	97957..98391	-	435	WP_000405672.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / catA2 / aadA2 / qacE / sul1 / qnrA1 / dfrA19 / aph(3'')-Ib / aph(6)-Id / mcr-9 / tet(D) / aac(6')-IIc / ere(A) / sul2 / blaSHV-12	-	1..302836	302836
	-	flank	IS/Tn	-	-	96862..97866	1004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50333.32 Da
• Isoelectric Point: 6.5044

>T168841 WP_000088044.1 NZ_CP059425:94040-95365 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T168841 NZ_CP076245:2231549-2231652 [Escherichia coli O157:H7]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10860.30 Da
• Isoelectric Point: 10.3134

>AT168841 WP_012477377.1 NZ_CP059425:93742-94035 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

>AT168841 NZ_CP076245:c2231690-2231419 [Escherichia coli O157:H7]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A2S1XXW1

Antitoxin

• AlphaFold DB: A0A6M3PBR7