Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 16361..17004 Replicon plasmid p49790_IncFIB
Accession NZ_CP059424
Organism Enterobacter hormaechei strain ENCL49790

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag H1Z82_RS23715 Protein ID WP_001044770.1
Coordinates 16361..16777 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag H1Z82_RS23720 Protein ID WP_001261282.1
Coordinates 16774..17004 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1Z82_RS23690 11939..12205 - 267 WP_001567383.1 DUF1471 domain-containing protein -
H1Z82_RS23695 12579..13583 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
H1Z82_RS23700 13662..15245 - 1584 Protein_18 Tn3 family transposase -
H1Z82_RS23705 15300..16004 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
H1Z82_RS23710 16051..16287 - 237 WP_001348075.1 AAA family ATPase -
H1Z82_RS23715 16361..16777 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
H1Z82_RS23720 16774..17004 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H1Z82_RS23725 16961..17203 + 243 Protein_23 hypothetical protein -
H1Z82_RS23730 17505..17795 + 291 WP_013087126.1 hypothetical protein -
H1Z82_RS23735 17926..18456 + 531 WP_013087127.1 hypothetical protein -
H1Z82_RS23740 18475..19251 + 777 WP_015572054.1 site-specific integrase -
H1Z82_RS23745 19453..19710 + 258 Protein_27 site-specific integrase -
H1Z82_RS23750 19954..20964 - 1011 WP_015572055.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..119988 119988


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T168839 WP_001044770.1 NZ_CP059424:c16777-16361 [Enterobacter hormaechei]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T168839 NZ_CP076245:946563-946970 [Escherichia coli O157:H7]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT168839 WP_001261282.1 NZ_CP059424:c17004-16774 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT168839 NZ_CP076245:946316-946582 [Escherichia coli O157:H7]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References