Detailed information of TA system TA168838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4814439..4815055
• Replicon: chromosome
• Accession: NZ_CP059422
• Organism: Enterobacter hormaechei strain ENCL49790

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H1Z82_RS23360
• Protein ID: WP_045142560.1
• Coordinates: 4814439..4814810 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: H1Z82_RS23365
• Protein ID: WP_015569912.1
• Coordinates: 4814813..4815055 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1Z82_RS23345	4811939..4812841	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
H1Z82_RS23350	4812838..4813473	+	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
H1Z82_RS23355	4813470..4814399	+	930	WP_045142561.1	formate dehydrogenase accessory protein FdhE	-
H1Z82_RS23360	4814439..4814810	-	372	WP_045142560.1	type II toxin-antitoxin system VapC family toxin	Toxin
H1Z82_RS23365	4814813..4815055	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
H1Z82_RS23370	4815254..4816174	+	921	WP_045142559.1	alpha/beta hydrolase	-
H1Z82_RS23375	4816183..4817124	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
H1Z82_RS23380	4817169..4817606	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H1Z82_RS23385	4817603..4818484	-	882	WP_003861949.1	virulence factor BrkB family protein	-
H1Z82_RS23390	4818478..4819077	-	600	WP_003861947.1	glucose-1-phosphatase	-
H1Z82_RS23395	4819196..4819996	-	801	WP_045142558.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13716.82 Da
• Isoelectric Point: 6.6419

>T168838 WP_045142560.1 NZ_CP059422:c4814810-4814439 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRRGHEAKTAAVMGAFEIIDVSRDIAERSVSLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T168838 NZ_CP076245:751449-751763 [Escherichia coli O157:H7]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT168838 WP_015569912.1 NZ_CP059422:c4815055-4814813 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT168838 NZ_CP076245:751760-752176 [Escherichia coli O157:H7]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3