Detailed information of TA system TA168827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1758524..1759321
• Replicon: chromosome
• Accession: NZ_CP059422
• Organism: Enterobacter hormaechei strain ENCL49790

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: H1Z82_RS08380
• Protein ID: WP_045141441.1
• Coordinates: 1758800..1759321 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: F5RT92
• Locus tag: H1Z82_RS08375
• Protein ID: WP_001303459.1
• Coordinates: 1758524..1758793 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1Z82_RS08340	1754064..1754423	+	360	WP_017384779.1	helix-turn-helix transcriptional regulator	-
H1Z82_RS08345	1754433..1754597	-	165	WP_072044142.1	helix-turn-helix transcriptional regulator	-
H1Z82_RS08350	1754750..1755991	+	1242	WP_017693667.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
H1Z82_RS08355	1756024..1756251	-	228	WP_006809324.1	YccJ family protein	-
H1Z82_RS08360	1756270..1756866	-	597	WP_006809323.1	NAD(P)H:quinone oxidoreductase	-
H1Z82_RS08365	1757258..1757428	+	171	WP_001273664.1	general stress protein	-
H1Z82_RS08370	1757545..1758450	+	906	WP_045141440.1	DMT family transporter	-
H1Z82_RS08375	1758524..1758793	+	270	WP_001303459.1	DUF1778 domain-containing protein	Antitoxin
H1Z82_RS08380	1758800..1759321	+	522	WP_045141441.1	GNAT family N-acetyltransferase	Toxin
H1Z82_RS08385	1759380..1760702	-	1323	WP_045141442.1	pyrimidine utilization transport protein G	-
H1Z82_RS08390	1760724..1761215	-	492	WP_045141443.1	pyrimidine utilization flavin reductase protein F	-
H1Z82_RS08395	1761228..1761818	-	591	WP_062934089.1	malonic semialdehyde reductase	-
H1Z82_RS08400	1761828..1762628	-	801	WP_039271149.1	pyrimidine utilization protein D	-
H1Z82_RS08405	1762636..1763022	-	387	WP_015570870.1	pyrimidine utilization protein C	-
H1Z82_RS08410	1763034..1763723	-	690	WP_045141445.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-88)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19407.16 Da
• Isoelectric Point: 7.4318

>T168827 WP_045141441.1 NZ_CP059422:1758800-1759321 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLVRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTALVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Nucleotide

• Length: 522 bp

>T168827 NZ_CP076243:c4391715-4391317 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10248.70 Da
• Isoelectric Point: 5.8836

>AT168827 WP_001303459.1 NZ_CP059422:1758524-1758793 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT168827 NZ_CP076243:c4392011-4391718 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7D7BCZ2