Detailed information of TA system TA168823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 207729..208255
• Replicon: plasmid p48880_mcr
• Accession: NZ_CP059421
• Organism: Enterobacter hormaechei strain ENCL48880

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: H1D58_RS24350
• Protein ID: WP_000323025.1
• Coordinates: 207729..208016 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: H1D58_RS24355
• Protein ID: WP_000534858.1
• Coordinates: 208016..208255 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D58_RS24315	203299..203475	-	177	WP_001371930.1	hypothetical protein	-
H1D58_RS24320	203986..204930	+	945	WP_000778029.1	DUF5417 domain-containing protein	-
H1D58_RS24325	205029..205628	+	600	WP_001031420.1	hypothetical protein	-
H1D58_RS24330	205688..206038	+	351	WP_000743059.1	hypothetical protein	-
H1D58_RS24335	206085..206288	+	204	WP_001015183.1	hypothetical protein	-
H1D58_RS24340	206570..206890	+	321	WP_000332796.1	hypothetical protein	-
H1D58_RS24345	207499..207657	-	159	WP_001447866.1	type I toxin-antitoxin system Hok family toxin	-
H1D58_RS24350	207729..208016	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
H1D58_RS24355	208016..208255	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
H1D58_RS24360	208518..209441	-	924	WP_000167917.1	cation diffusion facilitator family transporter	-
H1D58_RS24365	209641..210213	-	573	WP_001515348.1	cytochrome b/b6 domain-containing protein	-
H1D58_RS24370	210689..211927	-	1239	WP_000219087.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaVIM-4 / aac(6')-Il / dfrA1 / aadA1 / qacE / sul1 / tet(A) / mcr-9	-	1..262616	262616

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T168823 WP_000323025.1 NZ_CP059421:c208016-207729 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T168823 NZ_CP076243:c3286238-3285864 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT168823 WP_000534858.1 NZ_CP059421:c208255-208016 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT168823 NZ_CP076243:c3286659-3286285 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8