Detailed information of TA system TA168811

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2420743..2421482
• Replicon: chromosome
• Accession: NZ_CP059419
• Organism: Enterobacter hormaechei strain ENCL48880

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: H1D58_RS12910
• Protein ID: WP_163347044.1
• Coordinates: 2420743..2421228 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: H1D58_RS12915
• Protein ID: WP_003857131.1
• Coordinates: 2421216..2421482 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D58_RS12885	2416895..2417215	-	321	WP_000941305.1	transcriptional regulator	-
H1D58_RS12890	2417302..2418006	+	705	WP_000130816.1	arsenical resistance protein ArsH	-
H1D58_RS12895	2418039..2419442	-	1404	WP_000125668.1	ISNCY family transposase	-
H1D58_RS12900	2419764..2420009	-	246	WP_163347006.1	hypothetical protein	-
H1D58_RS12905	2420143..2420717	+	575	Protein_2229	hypothetical protein	-
H1D58_RS12910	2420743..2421228	-	486	WP_163347044.1	GNAT family N-acetyltransferase	Toxin
H1D58_RS12915	2421216..2421482	-	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
H1D58_RS12920	2421546..2422475	-	930	WP_163347042.1	LysR family transcriptional regulator	-
H1D58_RS12925	2422605..2423993	+	1389	WP_163347040.1	MFS transporter	-
H1D58_RS12930	2423972..2424529	-	558	WP_023300131.1	OmpH family outer membrane protein	-
H1D58_RS12935	2424650..2425786	-	1137	WP_023300132.1	type 1 fimbrial protein	-
H1D58_RS12940	2425810..2426334	-	525	WP_087878309.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2418039..2419442	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17500.15 Da
• Isoelectric Point: 9.6275

>T168811 WP_163347044.1 NZ_CP059419:c2421228-2420743 [Enterobacter hormaechei]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNI
PDPIPVIILARLAVDVSIRGNGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T168811 NZ_CP076243:359391-359666 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT168811 WP_003857131.1 NZ_CP059419:c2421482-2421216 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT168811 NZ_CP076243:359663-360022 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9