Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 103786..104429 Replicon plasmid p48212_IncFIB
Accession NZ_CP059416
Organism Enterobacter hormaechei strain ENCL48212

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag H1D56_RS25855 Protein ID WP_001044770.1
Coordinates 104013..104429 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag H1D56_RS25850 Protein ID WP_001261282.1
Coordinates 103786..104016 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D56_RS25820 99826..100836 + 1011 WP_015572055.1 RepB family plasmid replication initiator protein -
H1D56_RS25825 101080..101337 - 258 Protein_118 site-specific integrase -
H1D56_RS25830 101539..102315 - 777 WP_015572054.1 site-specific integrase -
H1D56_RS25835 102334..102864 - 531 WP_013087127.1 hypothetical protein -
H1D56_RS25840 102995..103285 - 291 WP_013087126.1 hypothetical protein -
H1D56_RS25845 103587..103829 - 243 Protein_122 hypothetical protein -
H1D56_RS25850 103786..104016 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H1D56_RS25855 104013..104429 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
H1D56_RS25860 104503..104739 + 237 WP_001348075.1 AAA family ATPase -
H1D56_RS25865 104786..105490 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
H1D56_RS25870 105545..107128 + 1584 Protein_127 Tn3 family transposase -
H1D56_RS25875 107207..108211 + 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
H1D56_RS25880 108585..108851 + 267 WP_001567383.1 DUF1471 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..119967 119967


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T168806 WP_001044770.1 NZ_CP059416:104013-104429 [Enterobacter hormaechei]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T168806 NZ_CP076241:c4885287-4884937 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT168806 WP_001261282.1 NZ_CP059416:103786-104016 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT168806 NZ_CP076241:c4885532-4885281 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References