Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 71186..71925 Replicon plasmid p48212_IncFIB
Accession NZ_CP059416
Organism Enterobacter hormaechei strain ENCL48212

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag H1D56_RS25640 Protein ID WP_013087172.1
Coordinates 71186..71671 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag H1D56_RS25645 Protein ID WP_012540086.1
Coordinates 71659..71925 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D56_RS25610 66655..67218 - 564 WP_181757423.1 chlorite dismutase family protein -
H1D56_RS25615 67242..67616 - 375 WP_001348195.1 cupin domain-containing protein -
H1D56_RS25620 67681..68220 - 540 WP_181757427.1 transposase -
H1D56_RS25625 68486..68820 + 335 Protein_78 thermonuclease family protein -
H1D56_RS25630 69694..70398 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
H1D56_RS25635 70442..70768 - 327 Protein_80 helix-turn-helix domain containing protein -
H1D56_RS25640 71186..71671 - 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
H1D56_RS25645 71659..71925 - 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
H1D56_RS25650 72225..72548 + 324 WP_013087171.1 metalloregulator ArsR/SmtB family transcription factor -
H1D56_RS25655 72550..73263 + 714 Protein_84 arsenical resistance protein ArsH -
H1D56_RS25660 73272..73817 + 546 WP_013087169.1 sigma-70 family RNA polymerase sigma factor -
H1D56_RS25665 73893..74255 + 363 WP_013087168.1 arsenic metallochaperone ArsD family protein -
H1D56_RS25670 74282..76033 + 1752 WP_162875198.1 arsenical pump-driving ATPase -
H1D56_RS25675 76158..76694 + 537 WP_013087166.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..119967 119967


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T168805 WP_013087172.1 NZ_CP059416:c71671-71186 [Enterobacter hormaechei]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T168805 NZ_CP076241:4751875-4752216 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT168805 WP_012540086.1 NZ_CP059416:c71925-71659 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT168805 NZ_CP076241:c4751880-4751804 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References