Detailed information of TA system TA168804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4535427..4536023
• Replicon: chromosome
• Accession: NZ_CP059414
• Organism: Enterobacter hormaechei strain ENCL48212

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A7G3EX93
• Locus tag: H1D56_RS23645
• Protein ID: WP_015570164.1
• Coordinates: 4535427..4535729 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A927HL08
• Locus tag: H1D56_RS23650
• Protein ID: WP_023295755.1
• Coordinates: 4535736..4536023 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D56_RS23625	4531646..4532995	+	1350	WP_006810501.1	lysine-sensitive aspartokinase 3	-
H1D56_RS23630	4533089..4534033	+	945	WP_015570166.1	ketopantoate/pantoate/pantothenate transporter PanS	-
H1D56_RS23635	4534084..4534356	+	273	WP_017382612.1	DUF3811 domain-containing protein	-
H1D56_RS23640	4534357..4535229	-	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
H1D56_RS23645	4535427..4535729	+	303	WP_015570164.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H1D56_RS23650	4535736..4536023	+	288	WP_023295755.1	putative addiction module antidote protein	Antitoxin
H1D56_RS23655	4536020..4537651	-	1632	WP_003860350.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11425.18 Da
• Isoelectric Point: 10.3736

>T168804 WP_015570164.1 NZ_CP059414:4535427-4535729 [Enterobacter hormaechei]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRIYFKDQGNSIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T168804 NZ_CP076241:c4636022-4635708 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10101.59 Da
• Isoelectric Point: 5.7807

>AT168804 WP_023295755.1 NZ_CP059414:4535736-4536023 [Enterobacter hormaechei]
MHKLTPYDPANALLDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT168804 NZ_CP076241:c4636258-4636025 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A7G3EX93