Detailed information of TA system TA168788

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 53977..54593
• Replicon: chromosome
• Accession: NZ_CP059414
• Organism: Enterobacter hormaechei strain ENCL48212

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H1D56_RS01875
• Protein ID: WP_045142560.1
• Coordinates: 54222..54593 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: H1D56_RS01870
• Protein ID: WP_015569912.1
• Coordinates: 53977..54219 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D56_RS01840	49036..49836	+	801	WP_045142558.1	DeoR/GlpR family DNA-binding transcription regulator	-
H1D56_RS01845	49955..50554	+	600	WP_003861947.1	glucose-1-phosphatase	-
H1D56_RS01850	50548..51429	+	882	WP_003861949.1	virulence factor BrkB family protein	-
H1D56_RS01855	51426..51863	+	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H1D56_RS01860	51908..52849	+	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
H1D56_RS01865	52858..53778	-	921	WP_045142559.1	alpha/beta hydrolase	-
H1D56_RS01870	53977..54219	+	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
H1D56_RS01875	54222..54593	+	372	WP_045142560.1	type II toxin-antitoxin system VapC family toxin	Toxin
H1D56_RS01880	54633..55562	-	930	WP_045142561.1	formate dehydrogenase accessory protein FdhE	-
H1D56_RS01885	55559..56194	-	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
H1D56_RS01890	56191..57093	-	903	WP_014072386.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13716.82 Da
• Isoelectric Point: 6.6419

>T168788 WP_045142560.1 NZ_CP059414:54222-54593 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRRGHEAKTAAVMGAFEIIDVSRDIAERSVSLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T168788 NZ_CP076241:418045-418572 [Escherichia coli O157:H7]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT168788 WP_015569912.1 NZ_CP059414:53977-54219 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT168788 NZ_CP076241:417772-418038 [Escherichia coli O157:H7]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3