Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 215801..217424 Replicon plasmid p48212_MCR
Accession NZ_CP059413
Organism Enterobacter hormaechei strain ENCL48212

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag H1D56_RS01225 Protein ID WP_000088044.1
Coordinates 215801..217126 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag H1D56_RS01230 Protein ID WP_012477377.1
Coordinates 217131..217424 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D56_RS01200 212775..213209 + 435 WP_000405672.1 Cd(II)/Pb(II)-responsive transcriptional regulator -
H1D56_RS01205 213300..214304 + 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
H1D56_RS01210 214582..215010 - 429 Protein_240 hypothetical protein -
H1D56_RS01215 215023..215433 - 411 WP_000985909.1 hypothetical protein -
H1D56_RS01220 215534..215740 - 207 WP_000134171.1 hypothetical protein -
H1D56_RS01225 215801..217126 - 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
H1D56_RS01230 217131..217424 - 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
H1D56_RS01235 217825..219213 - 1389 WP_012695445.1 hypothetical protein -
H1D56_RS01240 219218..219715 - 498 WP_000340139.1 membrane protein -
H1D56_RS01245 219918..220574 - 657 WP_000462754.1 trypsin-like serine protease -
H1D56_RS01250 221233..221625 - 393 WP_000341066.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaSHV-12 / sul2 / sul1 / qacE / ere(A) / aac(6')-IIc / tet(D) / mcr-9 / aph(6)-Id / aph(3'')-Ib / dfrA19 / qnrA1 / aadA2 / catA2 / blaTEM-1B - 1..302551 302551
- flank IS/Tn - - 213300..214304 1004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T168787 WP_000088044.1 NZ_CP059413:c217126-215801 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T168787 NZ_CP076241:375341-375616 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT168787 WP_012477377.1 NZ_CP059413:c217424-217131 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT168787 NZ_CP076241:375613-375972 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References