Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 93428..93954 Replicon plasmid p48212_MCR
Accession NZ_CP059413
Organism Enterobacter hormaechei strain ENCL48212

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag H1D56_RS00545 Protein ID WP_000323025.1
Coordinates 93667..93954 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag H1D56_RS00540 Protein ID WP_000534858.1
Coordinates 93428..93667 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D56_RS00525 89756..90994 + 1239 WP_000219087.1 IS110 family transposase -
H1D56_RS00530 91470..92042 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
H1D56_RS00535 92242..93165 + 924 WP_000167917.1 cation diffusion facilitator family transporter -
H1D56_RS00540 93428..93667 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
H1D56_RS00545 93667..93954 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
H1D56_RS00550 94026..94184 + 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
H1D56_RS00555 94793..95113 - 321 WP_000332796.1 hypothetical protein -
H1D56_RS00560 95645..95995 - 351 WP_000743059.1 hypothetical protein -
H1D56_RS00565 96055..96657 - 603 WP_012695474.1 hypothetical protein -
H1D56_RS00570 96753..97697 - 945 WP_000778029.1 DUF5417 domain-containing protein -
H1D56_RS00575 98208..98384 + 177 WP_001371930.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaSHV-12 / sul2 / sul1 / qacE / ere(A) / aac(6')-IIc / tet(D) / mcr-9 / aph(6)-Id / aph(3'')-Ib / dfrA19 / qnrA1 / aadA2 / catA2 / blaTEM-1B - 1..302551 302551


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T168786 WP_000323025.1 NZ_CP059413:93667-93954 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T168786 NZ_CP076241:293163-293270 [Escherichia coli O157:H7]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT168786 WP_000534858.1 NZ_CP059413:93428-93667 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT168786 NZ_CP076241:c293115-293049 [Escherichia coli O157:H7]
GGTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References