Detailed information of TA system TA168785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3187221..3188138
• Replicon: chromosome
• Accession: NZ_CP059405
• Organism: Bacillus velezensis strain MV2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: H1Q60_RS16545
• Protein ID: WP_007407256.1
• Coordinates: 3187392..3188138 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: H1Q60_RS16540
• Protein ID: WP_003154807.1
• Coordinates: 3187221..3187391 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1Q60_RS16495	3182427..3182939	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
H1Q60_RS16500	3183052..3183711	+	660	WP_065180407.1	terminase	-
H1Q60_RS16505	3183609..3184076	+	468	Protein_3186	hypothetical protein	-
H1Q60_RS16510	3184089..3184460	+	372	WP_065180408.1	hypothetical protein	-
H1Q60_RS16515	3184465..3184662	+	198	WP_007610833.1	XkdX family protein	-
H1Q60_RS16520	3184719..3185480	+	762	WP_063636818.1	hypothetical protein	-
H1Q60_RS16525	3185532..3185795	+	264	WP_021493821.1	hemolysin XhlA family protein	-
H1Q60_RS16530	3185809..3186072	+	264	WP_003154813.1	phage holin	-
H1Q60_RS16535	3186086..3186964	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
H1Q60_RS16540	3187221..3187391	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
H1Q60_RS16545	3187392..3188138	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
H1Q60_RS16550	3188242..3189240	-	999	WP_017417610.1	inorganic phosphate transporter	-
H1Q60_RS16555	3189253..3189870	-	618	WP_003154804.1	DUF47 domain-containing protein	-
H1Q60_RS16560	3190157..3191473	-	1317	WP_007610842.1	amino acid permease	-
H1Q60_RS16565	3191795..3192745	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T168785 WP_007407256.1 NZ_CP059405:c3188138-3187392 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T168785 NZ_CP076240:c2388-1348 [Escherichia coli O157:H7]
ATGGCACAGAACCCGTTTAAAGCACTAAATATCAATATTGACAAGATTGAGTCTGCTCTGACGCAGAATGGCGTCACAAA
CTATTCCTCTAATGTAAAAAACGAAAGAGAAACTCACATATCTGGCACATATAAAGGAATAGACTTCTTAATAAAACTAA
TGCCATCAGGCGGAAATACCACTATCGGAAGAGCGTCTGGGCAAAATAACACTTACTTTGATGAAATCGCCTTGATTATA
AAAGAAAACTGTTTGTATTCAGACACAAAGAACTTTGAATACACCATTCCAAAATTCAGTGATGATGACAGGGCAAATCT
ATTTGAATTTCTTTCTGAAGAGGGGATAACAATAACAGAAGATAATAATAACGATCCTAATTGTAAACACCAATATATTA
TGACCACCAGCAATGGTGACAGGGTCAGGGCAAAAATTTACAAGCGCGGCTCTATTCAATTCCAAGGAAAATACCTTCAA
ATCGCGAGTTTGATTAACGATTTCATGTGCTCAATACTAAACATGAAAGAGATTGTCGAACAAAAAAATAAAGAATTTAA
TGTTGACATAAAAAAAGAAACTATTGAGTCCGAGTTGCATAGCAAACTACCAAAAAGCATCGATAAAATCCATGAAGATA
TCAAAAAACAGCTATCATGCTCGCTAATAATGAAAAAAATAGATGTCGAAATGGAAGATTACTCAACATACTGCTTCTCT
GCATTAAGAGCCATAGAAGGCTTTATATATCAAATACTTAATGATGTTTGCAATCCATCATCATCAAAGAACCTTGGCGA
ATACTTCACTGAAAACAAACCCAAATATATAATCAGAGAAATACACCAAGAAACTATAAATGGTGAAATAGCGGAAGTTT
TGTGTGAATGCTACACTTACTGGCATGAGAACAGGCATGGTTTGTTTCATATGAAACCAGGAATAGCTGACACGAAGACA
ATTAACAAATTAGAATCAATCGCAATCATCGATACCGTTTGCCAATTAATAGATGGTGGCGTAGCTAGGTTGAAATTATG
A

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT168785 WP_003154807.1 NZ_CP059405:c3187391-3187221 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT168785 NZ_CP076240:c1351-971 [Escherichia coli O157:H7]
ATGAAAAAAGATAAAAAATATCAAATAGAAGCAATAAAAAATAAAGATAAAACTTTATTTATTGTCTATGCTACTGATAT
TTATAGCCCGAGCGAATTTTTCTCAAAAATCGAATCCGACTTGAAGAAAAAGAAAAGCAAGGGTGATGTTTTTTTTGATT
TAATAATTCCTAACGGTGGAAAAAAAGATCGTTACGTCTATACGTCATTTAATGGCGAGAAGTTTTCAAGTTACACATTA
AACAAAGTTACGAAAACTGATGAATATAATGATTTATCTGAGCTCTCGGCTTCGTTCTTTAAAAAAAACTTTGATAAGAT
CAACGTAAACCTTCTATCCAAAGCCACATCATTTGCTTTGAAAAAAGGCATTCCAATATAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14