Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2366251..2366888 Replicon chromosome
Accession NZ_CP059405
Organism Bacillus velezensis strain MV2

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag H1Q60_RS12120 Protein ID WP_003156187.1
Coordinates 2366538..2366888 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag H1Q60_RS12115 Protein ID WP_003156188.1
Coordinates 2366251..2366532 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1Q60_RS12095 2362616..2363215 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
H1Q60_RS12100 2363308..2363673 + 366 WP_007609580.1 holo-ACP synthase -
H1Q60_RS12105 2363838..2364845 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
H1Q60_RS12110 2364962..2366131 + 1170 WP_104843106.1 alanine racemase -
H1Q60_RS12115 2366251..2366532 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
H1Q60_RS12120 2366538..2366888 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
H1Q60_RS12125 2367006..2367827 + 822 WP_025649964.1 STAS domain-containing protein -
H1Q60_RS12130 2367832..2368197 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
H1Q60_RS12135 2368200..2368601 + 402 WP_003156178.1 anti-sigma regulatory factor -
H1Q60_RS12140 2368613..2369620 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
H1Q60_RS12145 2369684..2370013 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
H1Q60_RS12150 2370010..2370492 + 483 WP_007609591.1 anti-sigma B factor RsbW -
H1Q60_RS12155 2370458..2371246 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
H1Q60_RS12160 2371246..2371848 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T168784 WP_003156187.1 NZ_CP059405:2366538-2366888 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T168784 NZ_CP076239:54017-54322 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT168784 WP_003156188.1 NZ_CP059405:2366251-2366532 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT168784 NZ_CP076239:53797-54015 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References