Detailed information of TA system TA168781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 656418..657874
• Replicon: chromosome
• Accession: NZ_CP059400
• Organism: Legionella sp. PC1000

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: FOLKNPGA_RS02810
• Protein ID: WP_182351658.1
• Coordinates: 657023..657874 (+)
• Length: 284 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: FOLKNPGA_RS02805
• Protein ID: WP_182352737.1
• Coordinates: 656418..657026 (+)
• Length: 203 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOLKNPGA_RS02795	651836..652597	-	762	WP_182351656.1	helix-turn-helix transcriptional regulator	-
FOLKNPGA_RS02800	652936..655650	+	2715	WP_182351657.1	hypothetical protein	-
FOLKNPGA_RS02805	656418..657026	+	609	WP_182352737.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
FOLKNPGA_RS02810	657023..657874	+	852	WP_182351658.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
FOLKNPGA_RS02815	657958..659865	-	1908	WP_182352738.1	DUF2779 domain-containing protein	-
FOLKNPGA_RS02820	659994..660431	-	438	WP_182351659.1	conjugal transfer protein TraM	-
FOLKNPGA_RS02825	660431..661156	-	726	WP_182351660.1	conjugal transfer protein TraL	-
FOLKNPGA_RS02830	661156..661500	-	345	WP_182351661.1	TraK family protein	-
FOLKNPGA_RS02835	661894..662247	+	354	WP_182351662.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	656093..700432	44339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-263)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (9-56)

Sequences

Toxin

Protein

• Length: 284 a.a.
• Molecular weight: 31688.62 Da
• Isoelectric Point: 6.2785

>T168781 WP_182351658.1 NZ_CP059400:657023-657874 [Legionella sp. PC1000]
MTKNIGESIRSRLKNIAVKEGSDFNAVLIRYGLERLLYRIGESEYSKQFLLKGALLFNLWYDMPHRPTKDIDLLGFGDND
LAYIKQTFSNICSISADDGISFLSESVTVDTIKKDGGYTGARVELFGELAKAKIKIQIDIGYGDAVTPGPIDAHYPVLLT
DLPAPKIRTYPIYTVIAEKLHAIALLGMANSRLKDYLDLYVLLGNEQIDNQVLAKAIQATFNRRGMDLLEALPIGLTDEF
ANDPSRESMWKAFLRKNELEQKPLSEVIAVIRNLIQVPYSLAK

Nucleotide

• Length: 852 bp

>T168781 NZ_CP076237:c4971827-4971477 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 203 a.a.
• Molecular weight: 22947.84 Da
• Isoelectric Point: 9.2749

>AT168781 WP_182352737.1 NZ_CP059400:656418-657026 [Legionella sp. PC1000]
MKTEATYKQKVLQMASKTGIVRMSDLTERGITRATISRLVSENKLEKLASGLYCLPEAEFSEKESLVIIASRVPQAVFCL
LTALQIHDLTTQLPRKVWIAMPKGSHAPKMDYPPLKMVQYSDEAFSEGVEIIESDNIKLRVYNRAKTIADCFKHRNKIGL
DVAIEALKEAYTKNKVTVDELWHYAKICRVANVMRPYIEAIQ

Nucleotide

• Length: 609 bp

>AT168781 NZ_CP076237:c4972072-4971821 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA