Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 27170..27813 Replicon plasmid pFF1003_1
Accession NZ_CP059392
Organism Klebsiella africana strain FF1003

Toxin (Protein)


Gene name vagD Uniprot ID A0A5Q9LNG2
Locus tag H1X61_RS25405 Protein ID WP_048983057.1
Coordinates 27397..27813 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID R4IT22
Locus tag H1X61_RS25400 Protein ID WP_016338373.1
Coordinates 27170..27400 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1X61_RS25370 22658..23413 - 756 WP_001568031.1 replication initiation protein RepE -
H1X61_RS25375 24159..24272 + 114 WP_187358162.1 hypothetical protein -
H1X61_RS25380 24401..24658 + 258 WP_148390518.1 hypothetical protein -
H1X61_RS25385 24716..25492 - 777 WP_148390519.1 site-specific integrase -
H1X61_RS25390 25489..26232 - 744 WP_065810654.1 hypothetical protein -
H1X61_RS25395 26283..26633 - 351 WP_000493378.1 hypothetical protein -
H1X61_RS25400 27170..27400 + 231 WP_016338373.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H1X61_RS25405 27397..27813 + 417 WP_048983057.1 type II toxin-antitoxin system VapC family toxin Toxin
H1X61_RS25410 27854..28731 - 878 Protein_31 restriction endonuclease -
H1X61_RS25415 29390..30667 + 1278 WP_016338369.1 HlyD family secretion protein -
H1X61_RS25420 30657..32765 + 2109 WP_016338368.1 peptidase domain-containing ABC transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..97925 97925


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15022.52 Da        Isoelectric Point: 9.2957

>T168775 WP_048983057.1 NZ_CP059392:27397-27813 [Klebsiella africana]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVK

Download         Length: 417 bp

>T168775 NZ_CP076237:3561554-3561841 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8751.77 Da        Isoelectric Point: 4.3533

>AT168775 WP_016338373.1 NZ_CP059392:27170-27400 [Klebsiella africana]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWSSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT168775 NZ_CP076237:3561363-3561554 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNG2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LN61

References