Detailed information of TA system TA168774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5213070..5213695
• Replicon: chromosome
• Accession: NZ_CP059391
• Organism: Klebsiella africana strain FF1003

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H1X61_RS24890
• Protein ID: WP_136031286.1
• Coordinates: 5213070..5213453 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: H1X61_RS24895
• Protein ID: WP_168435282.1
• Coordinates: 5213453..5213695 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1X61_RS24875	5210437..5211339	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
H1X61_RS24880	5211336..5211971	+	636	WP_008807902.1	formate dehydrogenase cytochrome b556 subunit	-
H1X61_RS24885	5211968..5212897	+	930	WP_136031288.1	formate dehydrogenase accessory protein FdhE	-
H1X61_RS24890	5213070..5213453	-	384	WP_136031286.1	type II toxin-antitoxin system VapC family toxin	Toxin
H1X61_RS24895	5213453..5213695	-	243	WP_168435282.1	ribbon-helix-helix domain-containing protein	Antitoxin
H1X61_RS24900	5213900..5214817	+	918	WP_203033413.1	alpha/beta hydrolase	-
H1X61_RS24905	5214831..5215772	-	942	WP_012543287.1	fatty acid biosynthesis protein FabY	-
H1X61_RS24910	5215817..5216254	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H1X61_RS24915	5216251..5217111	-	861	WP_203033414.1	virulence factor BrkB family protein	-
H1X61_RS24920	5217105..5217704	-	600	WP_203033415.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14361.64 Da
• Isoelectric Point: 8.0552

>T168774 WP_136031286.1 NZ_CP059391:c5213453-5213070 [Klebsiella africana]
MTCGSALFDTNILIDLFSGRREAKQALEARPPQNAISLITWMEVMVGAKKYHQEQRTRMALSAFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTRDFAGIPGVVTPYELHPE

Nucleotide

• Length: 384 bp

>T168774 NZ_CP076237:c3554608-3554453 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9551.82 Da
• Isoelectric Point: 4.2500

>AT168774 WP_168435282.1 NZ_CP059391:c5213695-5213453 [Klebsiella africana]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDQAKDIISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT168774 NZ_CP076237:3554620-3554678 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA