Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3988870..3989489 Replicon chromosome
Accession NZ_CP059391
Organism Klebsiella africana strain FF1003

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag H1X61_RS19120 Protein ID WP_002892050.1
Coordinates 3989271..3989489 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A223UF87
Locus tag H1X61_RS19115 Protein ID WP_025710692.1
Coordinates 3988870..3989244 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1X61_RS19105 3984025..3985218 + 1194 WP_136033418.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H1X61_RS19110 3985241..3988387 + 3147 WP_203033068.1 multidrug efflux RND transporter permease subunit AcrB -
H1X61_RS19115 3988870..3989244 + 375 WP_025710692.1 Hha toxicity modulator TomB Antitoxin
H1X61_RS19120 3989271..3989489 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
H1X61_RS19125 3989646..3990212 + 567 WP_203033069.1 maltose O-acetyltransferase -
H1X61_RS19130 3990205..3990312 - 108 Protein_3750 hypothetical protein -
H1X61_RS19135 3990349..3990819 + 471 WP_025710690.1 YlaC family protein -
H1X61_RS19140 3990794..3992245 - 1452 WP_203033070.1 PLP-dependent aminotransferase family protein -
H1X61_RS19145 3992346..3993044 + 699 WP_136033425.1 GNAT family N-acetyltransferase -
H1X61_RS19150 3993041..3993181 - 141 WP_136033621.1 type B 50S ribosomal protein L36 -
H1X61_RS19155 3993181..3993444 - 264 WP_080924893.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T168771 WP_002892050.1 NZ_CP059391:3989271-3989489 [Klebsiella africana]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T168771 NZ_CP076237:c3089983-3089807 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14398.04 Da        Isoelectric Point: 4.7295

>AT168771 WP_025710692.1 NZ_CP059391:3988870-3989244 [Klebsiella africana]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIDQVDEYL
DDTFTLFSNYGINSSDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT168771 NZ_CP076237:c3089761-3089345 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A223UF87

References