Detailed information of TA system TA168759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 740596..741371
• Replicon: chromosome
• Accession: NZ_CP059391
• Organism: Klebsiella africana strain FF1003

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: H1X61_RS03640
• Protein ID: WP_136031433.1
• Coordinates: 740886..741371 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A7U2ZSW0
• Locus tag: H1X61_RS03635
• Protein ID: WP_025710477.1
• Coordinates: 740596..740889 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1X61_RS03615	735803..736405	-	603	WP_203033616.1	short chain dehydrogenase	-
H1X61_RS03620	736503..737414	+	912	WP_203033617.1	LysR family transcriptional regulator	-
H1X61_RS03625	737415..738563	-	1149	WP_136031430.1	PLP-dependent aspartate aminotransferase family protein	-
H1X61_RS03630	738574..739949	-	1376	Protein_713	cystathionine beta-synthase	-
H1X61_RS03635	740596..740889	+	294	WP_025710477.1	DUF1778 domain-containing protein	Antitoxin
H1X61_RS03640	740886..741371	+	486	WP_136031433.1	GNAT family N-acetyltransferase	Toxin
H1X61_RS03645	742084..742335	-	252	WP_203033618.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17480.41 Da
• Isoelectric Point: 8.2962

>T168759 WP_136031433.1 NZ_CP059391:740886-741371 [Klebsiella africana]
MISAPEPLHAGHILTPFCSGVDSMDNWLKQRAMKNQLSGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVEKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLGDLVH
A

Nucleotide

• Length: 486 bp

>T168759 NZ_CP076237:365396-365671 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10990.61 Da
• Isoelectric Point: 8.6141

>AT168759 WP_025710477.1 NZ_CP059391:740596-740889 [Klebsiella africana]
MPAANSMTMKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPEPN
AALRKTMQTPAPWEQEK

Nucleotide

• Length: 294 bp

>AT168759 NZ_CP076237:365668-366027 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U2ZSW0