Detailed information of TA system TA168748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2209630..2211225
• Replicon: chromosome
• Accession: NZ_CP059376
• Organism: Ralstonia solanacearum strain FJAT15244-F8

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: H1A20_RS10320
• Protein ID: WP_086706272.1
• Coordinates: 2209888..2211225 (+)
• Length: 446 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0S4UDK3
• Locus tag: H1A20_RS10315
• Protein ID: WP_016725279.1
• Coordinates: 2209630..2209884 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1A20_RS10290	2205693..2206619	-	927	WP_016727232.1	muramoyltetrapeptide carboxypeptidase	-
H1A20_RS10295	2206616..2207167	-	552	WP_016727233.1	tRNA adenosine(34) deaminase TadA	-
H1A20_RS10300	2207187..2207663	-	477	WP_011001399.1	6-carboxytetrahydropterin synthase QueD	-
H1A20_RS10305	2207731..2208369	-	639	WP_020831956.1	7-carboxy-7-deazaguanine synthase	-
H1A20_RS10310	2208687..2209526	+	840	WP_016725278.1	oxaloacetate decarboxylase	-
H1A20_RS10315	2209630..2209884	+	255	WP_016725279.1	helix-turn-helix transcriptional regulator	Antitoxin
H1A20_RS10320	2209888..2211225	+	1338	WP_086706272.1	type II toxin-antitoxin system HipA family toxin	Toxin
H1A20_RS10325	2211222..2212424	+	1203	WP_011001394.1	MFS transporter	-
H1A20_RS10330	2212650..2213378	+	729	WP_021155798.1	DsbA family protein	-
H1A20_RS10335	2213391..2213954	-	564	WP_080624831.1	RNA 2',3'-cyclic phosphodiesterase	-
H1A20_RS10340	2214135..2214833	-	699	WP_016725453.1	protocatechuate 3,4-dioxygenase subunit alpha	-
H1A20_RS10345	2214830..2215570	-	741	WP_011001390.1	protocatechuate 3,4-dioxygenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-107)

Antitoxin

• Transcription regulator, HipB-like (10-66)

Sequences

Toxin

Protein

• Length: 446 a.a.
• Molecular weight: 48754.78 Da
• Isoelectric Point: 6.7232

>T168748 WP_086706272.1 NZ_CP059376:2209888-2211225 [Ralstonia solanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDGWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGTSKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Nucleotide

• Length: 1338 bp

>T168748 NZ_CP076235:c3484755-3484600 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 8754.07 Da
• Isoelectric Point: 10.4693

>AT168748 WP_016725279.1 NZ_CP059376:2209630-2209884 [Ralstonia solanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAGTPSAA
PADW

Nucleotide

• Length: 255 bp

>AT168748 NZ_CP076235:3484767-3484825 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin

• AlphaFold DB: A0A0S4UDK3