Detailed information of TA system TA168729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 966842..967486
• Replicon: chromosome
• Accession: NZ_CP059348
• Organism: Providencia rettgeri strain ZG2016M39-3

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: B6XA97
• Locus tag: H0913_RS04300
• Protein ID: WP_004905417.1
• Coordinates: 966842..967045 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: H0913_RS04305
• Protein ID: WP_154602723.1
• Coordinates: 967118..967486 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0913_RS04280	962705..964489	+	1785	WP_154637984.1	SmdB family multidrug efflux ABC transporter permease/ATP-binding protein	-
H0913_RS04285	964637..965503	-	867	WP_154637985.1	acyl-CoA thioesterase II	-
H0913_RS04290	965742..966200	+	459	WP_154637986.1	YbaY family lipoprotein	-
H0913_RS04300	966842..967045	-	204	WP_004905417.1	hemolysin expression modulator Hha	Toxin
H0913_RS04305	967118..967486	-	369	WP_154602723.1	Hha toxicity modulator TomB	Antitoxin
H0913_RS04310	968013..969392	-	1380	WP_154637987.1	murein transglycosylase D	-
H0913_RS04315	969475..970227	-	753	WP_154602721.1	hydroxyacylglutathione hydrolase	-
H0913_RS04320	970262..970987	+	726	WP_154602720.1	class I SAM-dependent methyltransferase	-
H0913_RS04325	971000..971470	-	471	WP_181478028.1	ribonuclease HI	-
H0913_RS04330	971525..972286	+	762	WP_132496201.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8061.34 Da
• Isoelectric Point: 6.9770

>T168729 WP_004905417.1 NZ_CP059348:c967045-966842 [Providencia rettgeri]
MTKSDYLMRLRKCTTIETLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPASVWKFVR

Nucleotide

• Length: 204 bp

>T168729 NZ_CP076232:5436044-5436508 [Escherichia coli O157:H7]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14113.99 Da
• Isoelectric Point: 4.4687

>AT168729 WP_154602723.1 NZ_CP059348:c967486-967118 [Providencia rettgeri]
MDEYSPKKHDIAELKYLCNSLNRDAISSLQKTNTHWVNDLSSPQSISLNELVEHIAAFVWRFKIKYPKENLVISLVEEYL
DETYNLFGSPVITFSEIIDWESMNQNLVAVLDDDLKCLTSKT

Nucleotide

• Length: 369 bp

>AT168729 NZ_CP076232:5435709-5436044 [Escherichia coli O157:H7]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A291E6Q2