Detailed information of TA system TA168728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 549806..550842
• Replicon: chromosome
• Accession: NZ_CP059348
• Organism: Providencia rettgeri strain ZG2016M39-3

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: H0913_RS02460
• Protein ID: WP_154602046.1
• Coordinates: 549806..550381 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: H0913_RS02465
• Protein ID: WP_154602047.1
• Coordinates: 550381..550842 (-)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0913_RS02440	545544..546575	+	1032	WP_154638652.1	carbon-nitrogen hydrolase family protein	-
H0913_RS02445	546629..547327	+	699	WP_181478658.1	Asp/Glu racemase	-
H0913_RS02450	547413..547865	+	453	WP_036955244.1	MarR family transcriptional regulator	-
H0913_RS02455	548140..549786	+	1647	WP_154602045.1	Na+/H+ antiporter	-
H0913_RS02460	549806..550381	-	576	WP_154602046.1	PIN domain-containing protein	Toxin
H0913_RS02465	550381..550842	-	462	WP_154602047.1	helix-turn-helix domain-containing protein	Antitoxin
H0913_RS02470	551173..551589	+	417	WP_181477934.1	nuclear transport factor 2 family protein	-
H0913_RS02475	551751..552038	+	288	WP_154602049.1	zinc ribbon domain-containing protein	-
H0913_RS02480	552075..552632	+	558	WP_154602050.1	methyltransferase	-
H0913_RS02485	552679..553299	-	621	WP_154602051.1	flavin reductase family protein	-
H0913_RS02490	553981..554325	+	345	WP_181478660.1	helix-turn-helix transcriptional regulator	-
H0913_RS02495	554457..555008	+	552	WP_154602053.1	type 1 fimbrial protein	-
H0913_RS02500	555083..555664	+	582	WP_181478662.1	type 1 fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21780.33 Da
• Isoelectric Point: 5.9236

>T168728 WP_154602046.1 NZ_CP059348:c550381-549806 [Providencia rettgeri]
MIHSPFPVVLDACVLYPSLLRDLLMHLAITGLYQAKWTDTIQDEWQRNLLSNRPDLAQKQLIRTASLMNNALPDARVSQY
EPLIEGIELPDLNDRHVVAAAIKCHAKIIVTLNLKDFPKESMDQLEIEALHPDEFISDLFDLNHALALSAVRRQRLSLTK
PEININEYFAALLRQGLPMTVKALEKYQSMI

Nucleotide

• Length: 576 bp

>T168728 NZ_CP076232:c5393638-5393324 [Escherichia coli O157:H7]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17032.65 Da
• Isoelectric Point: 6.4930

>AT168728 WP_154602047.1 NZ_CP059348:c550842-550381 [Providencia rettgeri]
MTHSILNSMSLPAPKEIEAAVRGQRELATYLSTKLETQQISIKDADDNIHQIELPTSALTLLMAILGELAAGNAVQVVPV
HAELTTQEAANILNVSRPHMVKLLEEGRLPFHKTGRHRRILFADLMQFKAQREKESLQAMQALVDQSQELGLY

Nucleotide

• Length: 462 bp

>AT168728 NZ_CP076232:c5393327-5392911 [Escherichia coli O157:H7]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA