Detailed information of TA system TA168727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 4332318..4333674
• Replicon: chromosome
• Accession: NZ_CP059347
• Organism: Providencia rettgeri strain 2353217

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: H0912_RS19715
• Protein ID: WP_102140047.1
• Coordinates: 4332709..4333674 (+)
• Length: 322 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: H0912_RS19710
• Protein ID: WP_102140046.1
• Coordinates: 4332318..4332722 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0912_RS19700	4328104..4331151	-	3048	WP_102140044.1	formate dehydrogenase-N subunit alpha	-
H0912_RS19705	4331326..4332156	+	831	WP_102140045.1	formate dehydrogenase accessory sulfurtransferase FdhD	-
H0912_RS19710	4332318..4332722	+	405	WP_102140046.1	S-adenosylhomocysteine hydrolase	Antitoxin
H0912_RS19715	4332709..4333674	+	966	WP_102140047.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
H0912_RS19720	4333746..4335110	-	1365	WP_102140048.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
H0912_RS19725	4335212..4336861	-	1650	WP_004906225.1	membrane protein insertase YidC	-
H0912_RS19730	4336864..4337124	-	261	WP_102140049.1	membrane protein insertion efficiency factor YidD	-
H0912_RS19735	4337088..4337447	-	360	WP_102140050.1	ribonuclease P protein component	-
H0912_RS19740	4337469..4337612	-	144	WP_042846776.1	50S ribosomal protein L34	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (39-300)

Antitoxin

• Protein of unknown function DUF6088 (6-131)

Sequences

Toxin

Protein

• Length: 322 a.a.
• Molecular weight: 37124.16 Da
• Isoelectric Point: 5.0044

>T168727 WP_102140047.1 NZ_CP059347:4332709-4333674 [Providencia rettgeri]
MKLDSSLFIDVADAIGLGNPAIVEKDYYVVQLLKLLSNFQFTYHQAIFSGGTALAKSDIKTYRMSEDIDIKLIPNQRYSE
LASRNARKTARREIKNIIEEIINQNSIFSLDNAPSVKDEYRYFSFDIRYPQDHRQAPCLRPFIKLEFIETDLLSEPISRN
IQSIYSNVTESDIEIYNMICSAIIDTQAEKLLSMLRRTASVARNGQREDDETLIRHVYDTFHIQLSQPSNIEFLSQLLKK
ALDGDITRYGNQHTQLVDSPITELRYGLKLLVETPKFIERYNNYVSPMVYAEVPTQWDEAIIAFTSLAHQVLDYIEQELN
K

Nucleotide

• Length: 966 bp

>T168727 NZ_CP076232:c5333544-5333164 [Escherichia coli O157:H7]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 15286.99 Da
• Isoelectric Point: 11.0636

>AT168727 WP_102140046.1 NZ_CP059347:4332318-4332722 [Providencia rettgeri]
MTIQDRIQKKVKRSKRSVFLRSDFSKIADYDQVGRILKKLTDNGLLMRIGYGVYVRARVNRITGKLMPDNPSGADGVIIE
VMKKLNVDYTFDKLSLMNLSGESTQIPSNIKIIPKNPKFTRKIKVGKQSINETR

Nucleotide

• Length: 405 bp

>AT168727 NZ_CP076232:c5334001-5333633 [Escherichia coli O157:H7]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACAACCGCCCCTGGTGGGGACTGCCCTGCACAGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGACGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGGCACTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA