Detailed information of TA system TA168721

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1605859..1606516
• Replicon: chromosome
• Accession: NZ_CP059347
• Organism: Providencia rettgeri strain 2353217

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: H0912_RS07035
• Protein ID: WP_102137433.1
• Coordinates: 1606106..1606516 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: D4C097
• Locus tag: H0912_RS07030
• Protein ID: WP_004261864.1
• Coordinates: 1605859..1606125 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0912_RS07015	1602859..1603755	-	897	WP_168222855.1	hypothetical protein	-
H0912_RS07020	1603996..1604616	-	621	WP_102137431.1	HD domain-containing protein	-
H0912_RS07025	1604628..1605611	-	984	WP_102137432.1	tRNA-modifying protein YgfZ	-
H0912_RS07030	1605859..1606125	+	267	WP_004261864.1	FAD assembly factor SdhE	Antitoxin
H0912_RS07035	1606106..1606516	+	411	WP_102137433.1	protein YgfX	Toxin
H0912_RS07040	1606627..1607145	-	519	WP_102137434.1	flavodoxin FldB	-
H0912_RS07045	1607263..1608165	+	903	WP_102137435.1	site-specific tyrosine recombinase XerD	-
H0912_RS07050	1608187..1608891	+	705	WP_102137788.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
H0912_RS07055	1608901..1610634	+	1734	WP_102137436.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-133)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15570.46 Da
• Isoelectric Point: 10.6521

>T168721 WP_102137433.1 NZ_CP059347:1606106-1606516 [Providencia rettgeri]
VVLWKSNLSISWKTQLFSTCVHGVVGMFLLLAPWSPDNSMIWLPLLVVVVASWAKSQKNISKIKGVAVLVNGNKVQWKKN
EWRILKAPWLSRYGVLLTLEALQGKPQKVHLWIAKDAVSEENWRNLNQLLLQYPDI

Nucleotide

• Length: 411 bp

>T168721 NZ_CP076232:c3222672-3222478 [Escherichia coli O157:H7]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10684.24 Da
• Isoelectric Point: 4.8942

>AT168721 WP_004261864.1 NZ_CP059347:1605859-1606125 [Providencia rettgeri]
MDIENKARIHWACRRGMRELDISIMPFFEYEYDDLTTHDKQVFVRLLECADPDLFNWLMNHGRPDDDELFRMIKLIQEKN
KNRGPVEI

Nucleotide

• Length: 267 bp

>AT168721 NZ_CP076232:3222301-3222479 [Escherichia coli O157:H7]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Antitoxin

• AlphaFold DB: A0A345M2Q5