Detailed information of TA system TA168717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 819199..819843
• Replicon: chromosome
• Accession: NZ_CP059346
• Organism: Providencia alcalifaciens strain 1701003

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: B6XA97
• Locus tag: H0910_RS03650
• Protein ID: WP_004905417.1
• Coordinates: 819199..819402 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: H0910_RS03655
• Protein ID: WP_181480603.1
• Coordinates: 819475..819843 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0910_RS03630	815093..816874	+	1782	WP_036967305.1	SmdB family multidrug efflux ABC transporter permease/ATP-binding protein	-
H0910_RS03635	817021..817887	-	867	WP_006662762.1	acyl-CoA thioesterase II	-
H0910_RS03640	818126..818584	+	459	WP_006662761.1	YbaY family lipoprotein	-
H0910_RS03650	819199..819402	-	204	WP_004905417.1	hemolysin expression modulator Hha	Toxin
H0910_RS03655	819475..819843	-	369	WP_181480603.1	Hha toxicity modulator TomB	Antitoxin
H0910_RS03660	820369..821748	-	1380	WP_036961894.1	murein transglycosylase D	-
H0910_RS03665	821831..822583	-	753	WP_006662758.1	hydroxyacylglutathione hydrolase	-
H0910_RS03670	822620..823345	+	726	WP_154632826.1	methyltransferase domain-containing protein	-
H0910_RS03675	823358..823828	-	471	WP_006657236.1	ribonuclease HI	-
H0910_RS03680	823883..824644	+	762	WP_006662757.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8061.34 Da
• Isoelectric Point: 6.9770

>T168717 WP_004905417.1 NZ_CP059346:c819402-819199 [Providencia alcalifaciens]
MTKSDYLMRLRKCTTIETLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPASVWKFVR

Nucleotide

• Length: 204 bp

>T168717 NZ_CP076232:c2495386-2495099 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14103.96 Da
• Isoelectric Point: 4.4687

>AT168717 WP_181480603.1 NZ_CP059346:c819843-819475 [Providencia alcalifaciens]
MDEYSPKKHDIAELKYLCNSLNRDAISSLQKTNTHWINDLSSSQSISLNELVEHIAAFVWRFKIKYPKENLVISLVEEYL
DETYNLFGSPVVTFSEIIDWESMNQNLVAVLDDDLKCLTSKT

Nucleotide

• Length: 369 bp

>AT168717 NZ_CP076232:c2495577-2495386 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA

Structures

Toxin

• AlphaFold DB: A0A291E6Q2