Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2917352..2917989 Replicon chromosome
Accession NZ_CP059344
Organism Bacillus velezensis strain K01

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag HU024_RS14210 Protein ID WP_003156187.1
Coordinates 2917352..2917702 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag HU024_RS14215 Protein ID WP_003156188.1
Coordinates 2917708..2917989 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HU024_RS14170 2912392..2912994 - 603 WP_007410234.1 SpoIIE family protein phosphatase -
HU024_RS14175 2912994..2913782 - 789 WP_032873003.1 RNA polymerase sigma factor SigB -
HU024_RS14180 2913748..2914230 - 483 WP_007609591.1 anti-sigma B factor RsbW -
HU024_RS14185 2914227..2914556 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
HU024_RS14190 2914620..2915627 - 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
HU024_RS14195 2915639..2916040 - 402 WP_003156178.1 anti-sigma regulatory factor -
HU024_RS14200 2916043..2916408 - 366 WP_003156180.1 RsbT antagonist protein RsbS -
HU024_RS14205 2916413..2917234 - 822 WP_003156182.1 STAS domain-containing protein -
HU024_RS14210 2917352..2917702 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HU024_RS14215 2917708..2917989 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
HU024_RS14220 2918109..2919278 - 1170 WP_032873001.1 alanine racemase -
HU024_RS14225 2919395..2920402 - 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
HU024_RS14230 2920567..2920932 - 366 WP_007609580.1 holo-ACP synthase -
HU024_RS14235 2921025..2921624 + 600 WP_032872997.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T168712 WP_003156187.1 NZ_CP059344:c2917702-2917352 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T168712 NZ_CP076232:c1252298-1251957 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT168712 WP_003156188.1 NZ_CP059344:c2917989-2917708 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT168712 NZ_CP076232:1252293-1252369 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References