Detailed information of TA system TA168711

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 2167815..2168732
• Replicon: chromosome
• Accession: NZ_CP059344
• Organism: Bacillus velezensis strain K01

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: HU024_RS10265
• Protein ID: WP_007407256.1
• Coordinates: 2167815..2168561 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: HU024_RS10270
• Protein ID: WP_003154807.1
• Coordinates: 2168562..2168732 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU024_RS10245	2163208..2164158	-	951	WP_032874618.1	ring-cleaving dioxygenase	-
HU024_RS10250	2164480..2165796	+	1317	WP_032874616.1	amino acid permease	-
HU024_RS10255	2166082..2166699	+	618	WP_032874614.1	DUF47 domain-containing protein	-
HU024_RS10260	2166712..2167710	+	999	WP_032874611.1	inorganic phosphate transporter	-
HU024_RS10265	2167815..2168561	+	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
HU024_RS10270	2168562..2168732	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
HU024_RS10275	2168989..2169867	-	879	WP_032874609.1	N-acetylmuramoyl-L-alanine amidase	-
HU024_RS10280	2169881..2170144	-	264	WP_003154813.1	phage holin	-
HU024_RS10285	2170158..2170421	-	264	WP_003154815.1	hemolysin XhlA family protein	-
HU024_RS10290	2170473..2171234	-	762	WP_032874607.1	hypothetical protein	-
HU024_RS10295	2171291..2171488	-	198	WP_032874605.1	XkdX family protein	-
HU024_RS10300	2171493..2171864	-	372	WP_032874603.1	YomQ/XkdW protein, phage-like element PBSX	-
HU024_RS10305	2171877..2173499	-	1623	WP_032874601.1	pyocin knob domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T168711 WP_007407256.1 NZ_CP059344:2167815-2168561 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T168711 NZ_CP076232:1118905-1119255 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT168711 WP_003154807.1 NZ_CP059344:2168562-2168732 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT168711 NZ_CP076232:1118660-1118911 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14