Detailed information of TA system TA168709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/COG1569-excise
• Location: 184740..185795
• Replicon: chromosome
• Accession: NZ_CP059343
• Organism: Kocuria varians strain 80

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CIB50_RS00855
• Protein ID: WP_094395018.1
• Coordinates: 185232..185795 (+)
• Length: 188 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: CIB50_RS00850
• Protein ID: WP_094395016.1
• Coordinates: 184740..185225 (+)
• Length: 162 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CIB50_RS00835	180108..181676	-	1569	WP_094395012.1	type I restriction-modification system subunit M	-
CIB50_RS00840	181892..183316	+	1425	WP_094395014.1	type I restriction endonuclease subunit R	-
CIB50_RS00845	183411..184619	+	1209	WP_094395238.1	DUF1624 domain-containing protein	-
CIB50_RS00850	184740..185225	+	486	WP_094395016.1	helix-turn-helix domain-containing protein	Antitoxin
CIB50_RS00855	185232..185795	+	564	WP_094395018.1	PIN domain-containing protein	Toxin
CIB50_RS00860	185869..187017	-	1149	WP_094395019.1	PLP-dependent cysteine synthase family protein	-
CIB50_RS00865	187061..187498	-	438	WP_012397587.1	cytidine deaminase	-
CIB50_RS00870	187696..187977	+	282	WP_094395021.1	hypothetical protein	-
CIB50_RS00875	188283..189491	+	1209	WP_094395023.1	TAXI family TRAP transporter solute-binding subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN family, putative toxin-antitoxin system toxin component (1-129)

Antitoxin

• Helix-turn-helix domain, group 17 (82-129)

Sequences

Toxin

Protein

• Length: 188 a.a.
• Molecular weight: 20889.05 Da
• Isoelectric Point: 5.3200

>T168709 WP_094395018.1 NZ_CP059343:185232-185795 [Kocuria varians]
MSFPVVLDACVLIPMPIADLLLRLATAKQFRPLWSDEILNEVERNLVKNLHKDPHSARRRVNAMRGFFPDALVENYESLV
PAMTNDPKDRHVLAAAVRSRAELIVTSNQKDFPTTALKPYDVAVRSPDDFLLDQLDLDPSTVVRIAEEVVADMRNPTITW
NGYLEGLARAGLPLFATELEKRTAGVG

Nucleotide

• Length: 564 bp

>T168709 NZ_CP076232:c473332-472847 [Escherichia coli O157:H7]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 162 a.a.
• Molecular weight: 17450.77 Da
• Isoelectric Point: 7.0748

>AT168709 WP_094395016.1 NZ_CP059343:184740-185225 [Kocuria varians]
MTTAPLDRSTVAPTPAHEEELKALAAFLEAAPSAQGRGIPYLVSPTGATQQIPAEVYSLLTHVVDALANNRGVSVVPTNA
KLTTQQAADHLGISRPTLVKLLEQGDIPFTKVGRHRRVLLTDLIAYEERLEKTRREGLRQATHEAANHGTYFDIPADPAT
R

Nucleotide

• Length: 486 bp

>AT168709 NZ_CP076232:c473601-473323 [Escherichia coli O157:H7]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA