Detailed information of TA system TA168708

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 2517103..2517628
• Replicon: chromosome
• Accession: NZ_CP059331
• Organism: Mannheimia haemolytica strain 0597

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: H1C99_RS12375
• Protein ID: WP_240030073.1
• Coordinates: 2517356..2517628 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A248ZY54
• Locus tag: H1C99_RS12370
• Protein ID: WP_006250323.1
• Coordinates: 2517103..2517369 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1C99_RS12350 (H1C99_12335)	2512583..2513623	-	1041	WP_020824044.1	IS481 family transposase	-
H1C99_RS12355 (H1C99_12340)	2513957..2515342	-	1386	WP_020824252.1	GTPase HflX	-
H1C99_RS12360 (H1C99_12345)	2515362..2515634	-	273	WP_006250326.1	RNA chaperone Hfq	-
H1C99_RS12365 (H1C99_12350)	2515703..2516656	-	954	WP_240021449.1	tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA	-
H1C99_RS12370 (H1C99_12355)	2517103..2517369	+	267	WP_006250323.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
H1C99_RS12375 (H1C99_12360)	2517356..2517628	+	273	WP_240030073.1	type II toxin-antitoxin system YafQ family toxin	Toxin
H1C99_RS12380 (H1C99_12365)	2517647..2519530	-	1884	WP_240030074.1	DNA mismatch repair endonuclease MutL	-
H1C99_RS12385 (H1C99_12370)	2519814..2521055	+	1242	WP_240021451.1	glutamate-5-semialdehyde dehydrogenase	-
H1C99_RS12390 (H1C99_12375)	2521057..2521902	+	846	WP_006251985.1	virulence factor BrkB family protein	-
H1C99_RS12395 (H1C99_12380)	2521877..2522311	+	435	WP_006250318.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2512583..2513623	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10562.10 Da
• Isoelectric Point: 6.4732

>T168708 WP_240030073.1 NZ_CP059331:2517356-2517628 [Mannheimia haemolytica]
MREIEYSNAFKRDYKKCAVESLSNSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELL
RIGSHSHLFG

Nucleotide

• Length: 273 bp

>T168708 NZ_CP076232:424190-424717 [Escherichia coli O157:H7]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9738.10 Da
• Isoelectric Point: 4.7954

>AT168708 WP_006250323.1 NZ_CP059331:2517103-2517369 [Mannheimia haemolytica]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADL
MEQLNARD

Nucleotide

• Length: 267 bp

>AT168708 NZ_CP076232:423917-424183 [Escherichia coli O157:H7]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A248ZY54