Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hicAB/HicB(antitoxin) |
| Location | 2264899..2265539 | Replicon | chromosome |
| Accession | NZ_CP059331 | ||
| Organism | Mannheimia haemolytica strain 0597 | ||
Toxin (Protein)
| Gene name | hicA | Uniprot ID | A0A378NFX7 |
| Locus tag | H1C99_RS11005 | Protein ID | WP_006250075.1 |
| Coordinates | 2264899..2265081 (+) | Length | 61 a.a. |
Antitoxin (Protein)
| Gene name | hicB | Uniprot ID | A0A378NET2 |
| Locus tag | H1C99_RS11010 | Protein ID | WP_006250984.1 |
| Coordinates | 2265120..2265539 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| H1C99_RS10970 (H1C99_10965) | 2260466..2261512 | - | 1047 | WP_240029997.1 | conserved phage C-terminal domain-containing protein | - |
| H1C99_RS10975 (H1C99_10970) | 2261509..2262348 | - | 840 | WP_006252350.1 | Rha family transcriptional regulator | - |
| H1C99_RS10980 (H1C99_10975) | 2262523..2262783 | - | 261 | WP_240028402.1 | CII family transcriptional regulator | - |
| H1C99_RS10985 (H1C99_10980) | 2262804..2263004 | - | 201 | WP_006248825.1 | YdaS family helix-turn-helix protein | - |
| H1C99_RS10990 (H1C99_10985) | 2263135..2263824 | + | 690 | WP_240029998.1 | S24 family peptidase | - |
| H1C99_RS10995 (H1C99_10990) | 2263899..2264279 | + | 381 | WP_006248823.1 | hypothetical protein | - |
| H1C99_RS11000 | 2264284..2264502 | + | 219 | WP_240029999.1 | hypothetical protein | - |
| H1C99_RS11005 (H1C99_11000) | 2264899..2265081 | + | 183 | WP_006250075.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
| H1C99_RS11010 (H1C99_11005) | 2265120..2265539 | + | 420 | WP_006250984.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
| H1C99_RS11015 (H1C99_11010) | 2265606..2265911 | - | 306 | WP_006250985.1 | HigA family addiction module antitoxin | - |
| H1C99_RS11020 (H1C99_11015) | 2265920..2266195 | - | 276 | WP_006250986.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| H1C99_RS11025 (H1C99_11020) | 2266484..2266714 | + | 231 | WP_006250987.1 | hypothetical protein | - |
| H1C99_RS11030 (H1C99_11025) | 2267193..2267516 | - | 324 | WP_006250988.1 | hypothetical protein | - |
| H1C99_RS11035 (H1C99_11030) | 2267709..2267894 | + | 186 | WP_020824222.1 | hypothetical protein | - |
| H1C99_RS11040 (H1C99_11035) | 2267878..2268042 | + | 165 | WP_020824223.1 | hypothetical protein | - |
| H1C99_RS11045 (H1C99_11040) | 2268048..2268506 | + | 459 | WP_240028132.1 | single-stranded DNA-binding protein | - |
| H1C99_RS11050 (H1C99_11045) | 2268542..2268901 | + | 360 | WP_006250991.1 | hypothetical protein | - |
| H1C99_RS11055 (H1C99_11050) | 2268973..2269776 | + | 804 | WP_020824224.1 | DUF2303 family protein | - |
| H1C99_RS11060 (H1C99_11055) | 2269869..2270303 | + | 435 | WP_020824225.1 | DUF6378 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 2221627..2275385 | 53758 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 61 a.a. Molecular weight: 6933.03 Da Isoelectric Point: 10.3446
>T168707 WP_006250075.1 NZ_CP059331:2264899-2265081 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK
Download Length: 183 bp
>T168707 NZ_CP076232:381487-381762 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15957.34 Da Isoelectric Point: 4.6983
>AT168707 WP_006250984.1 NZ_CP059331:2265120-2265539 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH
Download Length: 420 bp
>AT168707 NZ_CP076232:381759-382118 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A378NFX7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A378NET2 |