Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 1108459..1108981 Replicon chromosome
Accession NZ_CP059331
Organism Mannheimia haemolytica strain 0597

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag H1C99_RS05515 Protein ID WP_006249269.1
Coordinates 1108459..1108755 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A378NDF7
Locus tag H1C99_RS05520 Protein ID WP_006250574.1
Coordinates 1108739..1108981 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1C99_RS05500 (H1C99_05485) 1103512..1104837 + 1326 WP_020831251.1 anti-phage deoxyguanosine triphosphatase -
H1C99_RS05505 (H1C99_05490) 1104900..1105259 + 360 WP_240018399.1 hypothetical protein -
H1C99_RS05510 (H1C99_05495) 1105420..1108332 + 2913 WP_240028743.1 RNA polymerase-associated protein RapA -
H1C99_RS05515 (H1C99_05500) 1108459..1108755 + 297 WP_006249269.1 BrnT family toxin Toxin
H1C99_RS05520 (H1C99_05505) 1108739..1108981 + 243 WP_006250574.1 hypothetical protein Antitoxin
H1C99_RS05525 (H1C99_05510) 1108993..1109685 + 693 WP_240030359.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
H1C99_RS05530 (H1C99_05515) 1109610..1110214 - 605 Protein_1056 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
H1C99_RS05535 (H1C99_05520) 1110274..1111116 + 843 WP_006250575.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
H1C99_RS05540 (H1C99_05525) 1111239..1111880 + 642 WP_006250576.1 orotate phosphoribosyltransferase -
H1C99_RS05545 (H1C99_05530) 1112052..1113131 + 1080 WP_240018396.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T168706 WP_006249269.1 NZ_CP059331:1108459-1108755 [Mannheimia haemolytica]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

>T168706 NZ_CP076232:299327-299434 [Escherichia coli O157:H7]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9320.71 Da        Isoelectric Point: 7.1665

>AT168706 WP_006250574.1 NZ_CP059331:1108739-1108981 [Mannheimia haemolytica]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALSKMWIAEKLPVQSPR

Download         Length: 243 bp

>AT168706 NZ_CP076232:c299279-299213 [Escherichia coli O157:H7]
GGTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A378NDF7

References