Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 55487..56076 Replicon chromosome
Accession NZ_CP059331
Organism Mannheimia haemolytica strain 0597

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag H1C99_RS00310 Protein ID WP_006248516.1
Coordinates 55744..56076 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag H1C99_RS00305 Protein ID WP_006248517.1
Coordinates 55487..55747 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1C99_RS00280 (H1C99_00260) 50662..51807 - 1146 WP_006251102.1 FMN-dependent L-lactate dehydrogenase LldD -
H1C99_RS00285 (H1C99_00265) 52422..53072 + 651 WP_240020299.1 RNA pyrophosphohydrolase -
H1C99_RS00290 (H1C99_00270) 53069..53857 + 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
H1C99_RS00295 (H1C99_00275) 53865..54662 + 798 WP_006251103.1 prolipoprotein diacylglyceryl transferase -
H1C99_RS00300 (H1C99_00280) 54749..55417 + 669 WP_240020300.1 phosphoglycolate phosphatase -
H1C99_RS00305 (H1C99_00285) 55487..55747 + 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H1C99_RS00310 (H1C99_00290) 55744..56076 + 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
H1C99_RS00315 (H1C99_00295) 56203..57234 + 1032 WP_240030109.1 tryptophan--tRNA ligase -
H1C99_RS00320 (H1C99_00300) 57297..58052 - 756 WP_240028273.1 M48 family metallopeptidase -
H1C99_RS00325 (H1C99_00305) 58108..58635 - 528 WP_006248512.1 inorganic diphosphatase -
H1C99_RS00345 (H1C99_00325) 59111..60247 + 1137 WP_236610598.1 endolytic transglycosylase MltG -
H1C99_RS00350 (H1C99_00330) 60258..60878 + 621 WP_006248508.1 dTMP kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T168702 WP_006248516.1 NZ_CP059331:55744-56076 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

>T168702 NZ_CP076230:c4987805-4987455 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT168702 WP_006248517.1 NZ_CP059331:55487-55747 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp

>AT168702 NZ_CP076230:c4988050-4987799 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References