Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 5724..6372 Replicon chromosome
Accession NZ_CP059331
Organism Mannheimia haemolytica strain 0597

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag H1C99_RS00065 Protein ID WP_006251221.1
Coordinates 6196..6372 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag H1C99_RS00060 Protein ID WP_006251222.1
Coordinates 5724..6140 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1C99_RS00020 2047..2274 + 228 WP_240030096.1 hypothetical protein -
H1C99_RS00025 (H1C99_00005) 2196..2480 + 285 WP_240030097.1 hypothetical protein -
H1C99_RS00030 (H1C99_00010) 2495..3481 + 987 WP_240028255.1 encapsulin -
H1C99_RS00035 (H1C99_00015) 3493..3726 + 234 WP_075271287.1 hypothetical protein -
H1C99_RS00040 4281..4451 + 171 WP_021280623.1 hypothetical protein -
H1C99_RS00045 (H1C99_00030) 4453..4821 + 369 WP_006251225.1 hypothetical protein -
H1C99_RS00050 (H1C99_00035) 4818..5198 + 381 WP_006251224.1 hypothetical protein -
H1C99_RS00055 (H1C99_00040) 5202..5684 + 483 WP_006251223.1 phage tail tube protein -
H1C99_RS00060 (H1C99_00045) 5724..6140 - 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H1C99_RS00065 (H1C99_00050) 6196..6372 - 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
H1C99_RS00070 (H1C99_00055) 6474..7139 + 666 WP_015587050.1 DUF6246 family protein -
H1C99_RS00075 (H1C99_00060) 7558..8016 + 459 WP_240030098.1 hypothetical protein -
H1C99_RS00080 (H1C99_00065) 8083..8478 + 396 WP_006251217.1 hypothetical protein -
H1C99_RS00085 (H1C99_00070) 8564..11062 + 2499 WP_240030099.1 phage tail length tape measure family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 684..20046 19362


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T168701 WP_006251221.1 NZ_CP059331:c6372-6196 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T168701 NZ_CP076230:4854393-4854734 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT168701 WP_006251222.1 NZ_CP059331:c6140-5724 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT168701 NZ_CP076230:c4854398-4854322 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References