Detailed information of TA system TA168695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 190338..190860
• Replicon: chromosome
• Accession: NZ_CP059330
• Organism: Mannheimia haemolytica strain 0722

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: H1D00_RS01090
• Protein ID: WP_240017651.1
• Coordinates: 190591..190860 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A248ZY54
• Locus tag: H1D00_RS01085
• Protein ID: WP_006250323.1
• Coordinates: 190338..190604 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D00_RS01065 (H1D00_01085)	185814..186854	-	1041	WP_020824044.1	IS481 family transposase	-
H1D00_RS01070 (H1D00_01090)	187190..188578	-	1389	WP_240021448.1	GTPase HflX	-
H1D00_RS01075 (H1D00_01095)	188598..188870	-	273	WP_006250326.1	RNA chaperone Hfq	-
H1D00_RS01080 (H1D00_01100)	188939..189892	-	954	WP_240021449.1	tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA	-
H1D00_RS01085 (H1D00_01105)	190338..190604	+	267	WP_006250323.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
H1D00_RS01090 (H1D00_01110)	190591..190860	+	270	WP_240017651.1	type II toxin-antitoxin system YafQ family toxin	Toxin
H1D00_RS01095 (H1D00_01115)	190879..192762	-	1884	WP_240021450.1	DNA mismatch repair endonuclease MutL	-
H1D00_RS01100 (H1D00_01120)	193046..194287	+	1242	WP_240021451.1	glutamate-5-semialdehyde dehydrogenase	-
H1D00_RS01105 (H1D00_01125)	194289..195134	+	846	WP_006251985.1	virulence factor BrkB family protein	-
H1D00_RS01110 (H1D00_01130)	195109..195543	+	435	WP_006250318.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	185814..186854	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-88)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10447.99 Da
• Isoelectric Point: 6.4732

>T168695 WP_240017651.1 NZ_CP059330:190591-190860 [Mannheimia haemolytica]
MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLR
IGSHSHLFG

Nucleotide

• Length: 270 bp

>T168695 NZ_CP076230:c3583971-3583597 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9738.10 Da
• Isoelectric Point: 4.7954

>AT168695 WP_006250323.1 NZ_CP059330:190338-190604 [Mannheimia haemolytica]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADL
MEQLNARD

Nucleotide

• Length: 267 bp

>AT168695 NZ_CP076230:c3584391-3584017 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A248ZY54