Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2186339..2186935 Replicon chromosome
Accession NZ_CP059329
Organism Mannheimia haemolytica strain 0727

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag H1D01_RS11025 Protein ID WP_006249776.1
Coordinates 2186711..2186935 (-) Length 75 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A547EKP0
Locus tag H1D01_RS11020 Protein ID WP_006249777.1
Coordinates 2186339..2186668 (-) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D01_RS10995 (H1D01_11010) 2181784..2182473 + 690 WP_240025307.1 GTP cyclohydrolase I FolE -
H1D01_RS11000 (H1D01_11015) 2182537..2183322 + 786 WP_240025309.1 tRNA pseudouridine(38-40) synthase TruA -
H1D01_RS11005 (H1D01_11020) 2183374..2185572 - 2199 WP_240025311.1 copper-translocating P-type ATPase -
H1D01_RS11010 (H1D01_11025) 2185625..2185825 - 201 WP_006249779.1 heavy metal-associated domain-containing protein -
H1D01_RS11015 (H1D01_11030) 2185914..2186300 + 387 WP_240025317.1 Cu(I)-responsive transcriptional regulator -
H1D01_RS11020 (H1D01_11035) 2186339..2186668 - 330 WP_006249777.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H1D01_RS11025 (H1D01_11040) 2186711..2186935 - 225 WP_006249776.1 type II toxin-antitoxin system HicA family toxin Toxin
H1D01_RS11030 (H1D01_11045) 2187143..2189050 + 1908 WP_240025319.1 DNA topoisomerase IV subunit B -
H1D01_RS11035 (H1D01_11050) 2189124..2189729 - 606 WP_006249773.1 glycerol-3-phosphate 1-O-acyltransferase PlsY -
H1D01_RS11040 (H1D01_11055) 2189799..2190154 + 356 Protein_2143 dihydroneopterin aldolase -
H1D01_RS11045 (H1D01_11060) 2190151..2191044 + 894 Protein_2144 DMT family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(28-73)

Antitoxin

Uncharacterised protein family HicB

(55-105)


Sequences

Toxin        


Download         Length: 75 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 10.5597

>T168693 WP_006249776.1 NZ_CP059329:c2186935-2186711 [Mannheimia haemolytica]
MVVKILKRKSQKTLQQIYTQPISANIKWTNIEALFLELGAEIEEREGSRIAVVLFQQVRVFHRPHPNPDTDKGR

Download         Length: 225 bp

>T168693 NZ_CP076230:3102823-3102930 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 12312.06 Da        Isoelectric Point: 4.7022

>AT168693 WP_006249777.1 NZ_CP059329:c2186668-2186339 [Mannheimia haemolytica]
MRNIMDINGYKAVIAYDPETELFRGEFIGLNGGADFYADNVIQLKKEGELSLNIFLELCREKGIEPFKQYSGKFNVRLSP
ELHKAAVIAATAENLSLNEWINQTLEKSV

Download         Length: 330 bp

>AT168693 NZ_CP076230:c3102770-3102709 [Escherichia coli O157:H7]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A547EKP0

References