168692

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-RelB
Location 1675981..1676503 Replicon chromosome
Accession NZ_CP059329
Organism Mannheimia haemolytica strain 0727

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZX63
Locus tag H1D01_RS08485 Protein ID WP_006250322.1
Coordinates 1676234..1676503 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A248ZY54
Locus tag H1D01_RS08480 Protein ID WP_006250323.1
Coordinates 1675981..1676247 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D01_RS08460 (H1D01_08495) 1672841..1674229 - 1389 WP_240024957.1 GTPase HflX -
H1D01_RS13935 (H1D01_08500) 1674249..1674520 - 272 Protein_1645 RNA chaperone Hfq -
H1D01_RS08475 (H1D01_08505) 1674589..1675542 - 954 WP_240024960.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
H1D01_RS08480 (H1D01_08510) 1675981..1676247 + 267 WP_006250323.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
H1D01_RS08485 (H1D01_08515) 1676234..1676503 + 270 WP_006250322.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
H1D01_RS08490 (H1D01_08520) 1676522..1678405 - 1884 WP_240024961.1 DNA mismatch repair endonuclease MutL -
H1D01_RS08495 (H1D01_08525) 1678687..1679928 + 1242 WP_240024962.1 glutamate-5-semialdehyde dehydrogenase -
H1D01_RS08500 (H1D01_08530) 1679930..1680775 + 846 WP_006250319.1 virulence factor BrkB family protein -
H1D01_RS08505 (H1D01_08535) 1680750..1681184 + 435 WP_006250318.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-84)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10447.99 Da        Isoelectric Point: 6.4732

>T168692 WP_006250322.1 NZ_CP059329:1676234-1676503 [Mannheimia haemolytica]
MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLR
IGSHSHLFG

Download         Length: 270 bp

>T168692 NZ_CP076230:3102287-3102394 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9738.10 Da        Isoelectric Point: 4.7954

>AT168692 WP_006250323.1 NZ_CP059329:1675981-1676247 [Mannheimia haemolytica]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADL
MEQLNARD

Download         Length: 267 bp

>AT168692 NZ_CP076230:c3102240-3102180 [Escherichia coli O157:H7]
TGTCTGGTTTCAAGATTAGCCCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZX63

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZY54

References