Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | pemIK/PRK09812-MazE |
Location | 1632594..1633183 | Replicon | chromosome |
Accession | NZ_CP059329 | ||
Organism | Mannheimia haemolytica strain 0727 |
Toxin (Protein)
Gene name | pemK | Uniprot ID | A0A248ZY19 |
Locus tag | H1D01_RS08275 | Protein ID | WP_006248516.1 |
Coordinates | 1632594..1632926 (-) | Length | 111 a.a. |
Antitoxin (Protein)
Gene name | pemI | Uniprot ID | A0A248ZX26 |
Locus tag | H1D01_RS08280 | Protein ID | WP_006248517.1 |
Coordinates | 1632923..1633183 (-) | Length | 87 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
H1D01_RS08235 (H1D01_08275) | 1627785..1628408 | - | 624 | WP_240026339.1 | dTMP kinase | - |
H1D01_RS08240 (H1D01_08280) | 1628419..1629558 | - | 1140 | WP_240024938.1 | endolytic transglycosylase MltG | - |
H1D01_RS08260 (H1D01_08300) | 1630034..1630561 | + | 528 | WP_006248512.1 | inorganic diphosphatase | - |
H1D01_RS08265 (H1D01_08305) | 1630617..1631372 | + | 756 | WP_006248513.1 | M48 family metallopeptidase | - |
H1D01_RS08270 (H1D01_08310) | 1631437..1632468 | - | 1032 | WP_240017547.1 | tryptophan--tRNA ligase | - |
H1D01_RS08275 (H1D01_08315) | 1632594..1632926 | - | 333 | WP_006248516.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
H1D01_RS08280 (H1D01_08320) | 1632923..1633183 | - | 261 | WP_006248517.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
H1D01_RS08285 (H1D01_08325) | 1633253..1633924 | - | 672 | WP_240024939.1 | phosphoglycolate phosphatase | - |
H1D01_RS08290 (H1D01_08330) | 1634011..1634808 | - | 798 | WP_006248519.1 | prolipoprotein diacylglyceryl transferase | - |
H1D01_RS08295 (H1D01_08335) | 1634816..1635604 | - | 789 | WP_240017549.1 | sulfite exporter TauE/SafE family protein | - |
H1D01_RS08300 (H1D01_08340) | 1635606..1636250 | - | 645 | WP_240024940.1 | RNA pyrophosphohydrolase | - |
H1D01_RS08305 (H1D01_08345) | 1636863..1638011 | + | 1149 | WP_240024941.1 | FMN-dependent L-lactate dehydrogenase LldD | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 111 a.a. Molecular weight: 12007.94 Da Isoelectric Point: 6.4597
>T168691 WP_006248516.1 NZ_CP059329:c1632926-1632594 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE
Download Length: 333 bp
>T168691 NZ_CP076230:c2797609-2797433 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA
Antitoxin
Download Length: 87 a.a. Molecular weight: 9418.88 Da Isoelectric Point: 4.7228
>AT168691 WP_006248517.1 NZ_CP059329:c1633183-1632923 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL
Download Length: 261 bp
>AT168691 NZ_CP076230:c2797387-2796971 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A248ZY19 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A248ZX26 |