Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1632594..1633183 Replicon chromosome
Accession NZ_CP059329
Organism Mannheimia haemolytica strain 0727

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag H1D01_RS08275 Protein ID WP_006248516.1
Coordinates 1632594..1632926 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag H1D01_RS08280 Protein ID WP_006248517.1
Coordinates 1632923..1633183 (-) Length 87 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D01_RS08235 (H1D01_08275) 1627785..1628408 - 624 WP_240026339.1 dTMP kinase -
H1D01_RS08240 (H1D01_08280) 1628419..1629558 - 1140 WP_240024938.1 endolytic transglycosylase MltG -
H1D01_RS08260 (H1D01_08300) 1630034..1630561 + 528 WP_006248512.1 inorganic diphosphatase -
H1D01_RS08265 (H1D01_08305) 1630617..1631372 + 756 WP_006248513.1 M48 family metallopeptidase -
H1D01_RS08270 (H1D01_08310) 1631437..1632468 - 1032 WP_240017547.1 tryptophan--tRNA ligase -
H1D01_RS08275 (H1D01_08315) 1632594..1632926 - 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
H1D01_RS08280 (H1D01_08320) 1632923..1633183 - 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H1D01_RS08285 (H1D01_08325) 1633253..1633924 - 672 WP_240024939.1 phosphoglycolate phosphatase -
H1D01_RS08290 (H1D01_08330) 1634011..1634808 - 798 WP_006248519.1 prolipoprotein diacylglyceryl transferase -
H1D01_RS08295 (H1D01_08335) 1634816..1635604 - 789 WP_240017549.1 sulfite exporter TauE/SafE family protein -
H1D01_RS08300 (H1D01_08340) 1635606..1636250 - 645 WP_240024940.1 RNA pyrophosphohydrolase -
H1D01_RS08305 (H1D01_08345) 1636863..1638011 + 1149 WP_240024941.1 FMN-dependent L-lactate dehydrogenase LldD -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-109)

Antitoxin

(7-48)


Sequences


Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T168691 WP_006248516.1 NZ_CP059329:c1632926-1632594 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

>T168691 NZ_CP076230:c2797609-2797433 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT168691 WP_006248517.1 NZ_CP059329:c1633183-1632923 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp

>AT168691 NZ_CP076230:c2797387-2796971 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A248ZY19


Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References