Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 568299..568821 Replicon chromosome
Accession NZ_CP059329
Organism Mannheimia haemolytica strain 0727

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag H1D01_RS02995 Protein ID WP_006249269.1
Coordinates 568525..568821 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A249A190
Locus tag H1D01_RS02990 Protein ID WP_006249270.1
Coordinates 568299..568541 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D01_RS02965 (H1D01_02990) 564138..565229 - 1092 WP_240025933.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
H1D01_RS02970 (H1D01_02995) 565402..566043 - 642 WP_006249274.1 orotate phosphoribosyltransferase -
H1D01_RS02975 (H1D01_03000) 566165..567007 - 843 WP_240025934.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
H1D01_RS02980 (H1D01_03005) 567067..567641 + 575 Protein_594 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
H1D01_RS02985 (H1D01_03010) 567619..568287 - 669 WP_240025935.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
H1D01_RS02990 (H1D01_03015) 568299..568541 - 243 WP_006249270.1 CopG family antitoxin Antitoxin
H1D01_RS02995 (H1D01_03020) 568525..568821 - 297 WP_006249269.1 BrnT family toxin Toxin
H1D01_RS03000 (H1D01_03025) 568948..571860 - 2913 WP_240025936.1 RNA polymerase-associated protein RapA -
H1D01_RS03005 (H1D01_03030) 572020..572379 - 360 WP_240018399.1 hypothetical protein -
H1D01_RS03010 (H1D01_03035) 572442..573767 - 1326 WP_240025937.1 anti-phage deoxyguanosine triphosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - rfaE 383508..571161 187653


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T168688 WP_006249269.1 NZ_CP059329:c568821-568525 [Mannheimia haemolytica]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

>T168688 NZ_CP076230:1100599-1100934 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9346.79 Da        Isoelectric Point: 7.1665

>AT168688 WP_006249270.1 NZ_CP059329:c568541-568299 [Mannheimia haemolytica]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR

Download         Length: 243 bp

>AT168688 NZ_CP076230:1100351-1100599 [Escherichia coli O157:H7]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A249A190

References