Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hicAB/HicB(antitoxin) |
| Location | 257489..258129 | Replicon | chromosome |
| Accession | NZ_CP059329 | ||
| Organism | Mannheimia haemolytica strain 0727 | ||
Toxin (Protein)
| Gene name | hicA | Uniprot ID | A0A378NFX7 |
| Locus tag | H1D01_RS01375 | Protein ID | WP_006250075.1 |
| Coordinates | 257489..257671 (+) | Length | 61 a.a. |
Antitoxin (Protein)
| Gene name | hicB | Uniprot ID | A0A378NFW9 |
| Locus tag | H1D01_RS01380 | Protein ID | WP_006250074.1 |
| Coordinates | 257710..258129 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| H1D01_RS01345 (H1D01_01345) | 252624..252923 | + | 300 | WP_006247810.1 | XRE family transcriptional regulator | - |
| H1D01_RS01350 (H1D01_01350) | 253039..254079 | + | 1041 | WP_015587043.1 | IS481 family transposase | - |
| H1D01_RS01355 (H1D01_01355) | 254139..254297 | - | 159 | WP_006247809.1 | hypothetical protein | - |
| H1D01_RS01360 (H1D01_01365) | 255395..255595 | - | 201 | WP_006248825.1 | YdaS family helix-turn-helix protein | - |
| H1D01_RS01365 (H1D01_01370) | 255726..256412 | + | 687 | WP_240025799.1 | S24 family peptidase | - |
| H1D01_RS01370 (H1D01_01375) | 256487..256837 | + | 351 | WP_236964885.1 | hypothetical protein | - |
| H1D01_RS01375 (H1D01_01385) | 257489..257671 | + | 183 | WP_006250075.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
| H1D01_RS01380 (H1D01_01390) | 257710..258129 | + | 420 | WP_006250074.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
| H1D01_RS01385 (H1D01_01395) | 258463..259107 | + | 645 | WP_240025800.1 | tyrosine-type recombinase/integrase | - |
| H1D01_RS01390 (H1D01_01400) | 259244..260251 | + | 1008 | WP_240025801.1 | IS481 family transposase | - |
| H1D01_RS01395 (H1D01_01405) | 260318..261724 | - | 1407 | Protein_276 | YdgA family protein | - |
| H1D01_RS01400 (H1D01_01410) | 261772..262578 | - | 807 | WP_240025802.1 | tryptophan synthase subunit alpha | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 192459..315372 | 122913 | |
| - | flank | IS/Tn | - | - | 253039..254079 | 1040 | |
| - | flank | IS/Tn | - | - | 259244..260251 | 1007 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 61 a.a. Molecular weight: 6933.03 Da Isoelectric Point: 10.3446
>T168687 WP_006250075.1 NZ_CP059329:257489-257671 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK
Download Length: 183 bp
>T168687 NZ_CP076230:955117-955524 [Escherichia coli O157:H7]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15938.30 Da Isoelectric Point: 4.6963
>AT168687 WP_006250074.1 NZ_CP059329:257710-258129 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH
Download Length: 420 bp
>AT168687 NZ_CP076230:954870-955136 [Escherichia coli O157:H7]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A378NFX7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A378NFW9 |