Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1128753..1129401 Replicon chromosome
Accession NZ_CP059328
Organism Mannheimia haemolytica strain 0728

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag H1D02_RS05675 Protein ID WP_006251221.1
Coordinates 1129225..1129401 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag H1D02_RS05670 Protein ID WP_006251222.1
Coordinates 1128753..1129169 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1D02_RS05635 (H1D02_05675) 1125069..1125503 + 435 WP_006251230.1 hypothetical protein -
H1D02_RS05640 (H1D02_05680) 1125518..1126504 + 987 WP_240028255.1 encapsulin -
H1D02_RS05645 (H1D02_05685) 1126516..1126749 + 234 WP_075271287.1 hypothetical protein -
H1D02_RS05650 1127307..1127477 + 171 WP_021280623.1 hypothetical protein -
H1D02_RS05655 (H1D02_05700) 1127479..1127847 + 369 WP_006251225.1 hypothetical protein -
H1D02_RS05660 (H1D02_05705) 1127844..1128224 + 381 WP_240017575.1 hypothetical protein -
H1D02_RS05665 (H1D02_05710) 1128228..1128713 + 486 WP_240028256.1 phage tail tube protein -
H1D02_RS05670 (H1D02_05715) 1128753..1129169 - 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H1D02_RS05675 (H1D02_05720) 1129225..1129401 - 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
H1D02_RS05680 (H1D02_05725) 1129503..1130168 + 666 WP_015587050.1 DUF6246 family protein -
H1D02_RS05685 (H1D02_05735) 1130588..1131046 + 459 WP_006251218.1 hypothetical protein -
H1D02_RS05690 (H1D02_05740) 1131113..1131508 + 396 WP_006251217.1 hypothetical protein -
H1D02_RS05695 (H1D02_05745) 1131594..1134089 + 2496 WP_240028257.1 phage tail length tape measure family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1089278..1143077 53799


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T168681 WP_006251221.1 NZ_CP059328:c1129401-1129225 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T168681 NZ_CP076230:293156-293263 [Escherichia coli O157:H7]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT168681 WP_006251222.1 NZ_CP059328:c1129169-1128753 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT168681 NZ_CP076230:c293108-293042 [Escherichia coli O157:H7]
GGTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References