Detailed information of TA system TA168675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1665672..1666320
• Replicon: chromosome
• Accession: NZ_CP059327
• Organism: Mannheimia haemolytica strain 0729

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: H1D03_RS08305
• Protein ID: WP_006251221.1
• Coordinates: 1666144..1666320 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: H1D03_RS08300
• Protein ID: WP_006251222.1
• Coordinates: 1665672..1666088 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D03_RS08265 (H1D03_08290)	1661995..1662429	+	435	WP_006251230.1	hypothetical protein	-
H1D03_RS08270 (H1D03_08295)	1662444..1663430	+	987	WP_240028255.1	encapsulin	-
H1D03_RS08275 (H1D03_08300)	1663442..1663720	+	279	WP_240017576.1	hypothetical protein	-
H1D03_RS08280	1664229..1664399	+	171	WP_021280623.1	hypothetical protein	-
H1D03_RS08285 (H1D03_08315)	1664401..1664769	+	369	WP_006251225.1	hypothetical protein	-
H1D03_RS08290 (H1D03_08320)	1664766..1665146	+	381	WP_240017575.1	hypothetical protein	-
H1D03_RS08295 (H1D03_08325)	1665150..1665632	+	483	WP_006251223.1	phage tail tube protein	-
H1D03_RS08300 (H1D03_08330)	1665672..1666088	-	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H1D03_RS08305 (H1D03_08335)	1666144..1666320	-	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
H1D03_RS08310 (H1D03_08340)	1666423..1667088	+	666	WP_015587050.1	DUF6246 family protein	-
H1D03_RS08315 (H1D03_08345)	1667506..1667967	+	462	WP_240028489.1	hypothetical protein	-
H1D03_RS08320 (H1D03_08350)	1668034..1668429	+	396	WP_006251217.1	hypothetical protein	-
H1D03_RS08325 (H1D03_08355)	1668515..1671013	+	2499	WP_240028490.1	phage tail length tape measure family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1626211..1679997	53786

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T168675 WP_006251221.1 NZ_CP059327:c1666320-1666144 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T168675 NZ_CP076222:241541-241891 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT168675 WP_006251222.1 NZ_CP059327:c1666088-1665672 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT168675 NZ_CP076222:241249-241536 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6