Detailed information of TA system TA168674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 1519861..1520383
• Replicon: chromosome
• Accession: NZ_CP059327
• Organism: Mannheimia haemolytica strain 0729

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A248ZX63
• Locus tag: H1D03_RS07410
• Protein ID: WP_006250322.1
• Coordinates: 1519861..1520130 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A248ZY54
• Locus tag: H1D03_RS07415
• Protein ID: WP_006250323.1
• Coordinates: 1520117..1520383 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D03_RS07390 (H1D03_07400)	1515182..1515616	-	435	WP_006250318.1	D-aminoacyl-tRNA deacylase	-
H1D03_RS07395 (H1D03_07405)	1515591..1516436	-	846	WP_006251985.1	virulence factor BrkB family protein	-
H1D03_RS07400 (H1D03_07410)	1516438..1517676	-	1239	WP_240028449.1	glutamate-5-semialdehyde dehydrogenase	-
H1D03_RS07405 (H1D03_07415)	1517959..1519842	+	1884	WP_240028450.1	DNA mismatch repair endonuclease MutL	-
H1D03_RS07410 (H1D03_07420)	1519861..1520130	-	270	WP_006250322.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
H1D03_RS07415 (H1D03_07425)	1520117..1520383	-	267	WP_006250323.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
H1D03_RS07420 (H1D03_07430)	1520829..1521782	+	954	WP_006251982.1	tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA	-
H1D03_RS07425 (H1D03_07435)	1521851..1522123	+	273	WP_006250326.1	RNA chaperone Hfq	-
H1D03_RS07430 (H1D03_07440)	1522143..1523528	+	1386	WP_240028451.1	GTPase HflX	-
H1D03_RS07435 (H1D03_07445)	1523862..1524902	+	1041	WP_020824044.1	IS481 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1523862..1524902	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-88)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10447.99 Da
• Isoelectric Point: 6.4732

>T168674 WP_006250322.1 NZ_CP059327:c1520130-1519861 [Mannheimia haemolytica]
MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLR
IGSHSHLFG

Nucleotide

• Length: 270 bp

>T168674 NZ_CP076219:2302095-2302832 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9738.10 Da
• Isoelectric Point: 4.7954

>AT168674 WP_006250323.1 NZ_CP059327:c1520383-1520117 [Mannheimia haemolytica]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADL
MEQLNARD

Nucleotide

• Length: 267 bp

>AT168674 NZ_CP076219:2302945-2303070 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Toxin

• AlphaFold DB: A0A248ZX63

Antitoxin

• AlphaFold DB: A0A248ZY54